饮食习惯与日本人群肝癌风险的因果关系

Causal association between dietary habits and risk of liver cancer in Japan population

目的利用孟德尔随机化(MR)的方法探究不同的饮食行为与日本人群中肝癌发生的因果关联。方法从英国生物样本库(UK Biobank)中提取与不同饮食习惯显著相关的单核苷酸多态性(SNP)位点的相关信息(P<5e-6),并且剔除了连锁不平衡(LD r^(2)<0.001,kb=10000)、回文序列以及不兼容的SNP位点信息。用逆方差加权法(IVW)、MR-Egger法、加权中位数(Weighted median)、加权众数法(Weighted mode)、简单众数法(Simple mode)进行双样本MR分析。在敏感性分析中,Cochran's Q统计来检测异质性。采用MR-Egger回归、MR-Pleiotropy残差和离群点方法来检测多效性。结果分析了13种不同的饮食行为和日本人群中肝癌发生风险的因果关系。研究发现,面包摄入会增加日本人群肝癌的发病风险(IVW:OR:1.32,95%CI:1.09-1.60,P:0.004),但并没有得到由工具变量代理的其他饮食习惯和肝癌发生的因果关联。结论基于目前的数据,本研究认为面包摄入会增加日本人群肝癌的发病风险,但不能得出其他的饮食习惯和日本人群中肝癌的发生有因果关联。

Objective Mendelian randomization(MR)was used to investigate the causal association between different dietary behaviors and the occurrence of liver cancer in Japan population.Methods Information on single nucleotide polymorphisms(SNP)loci significantly associated with different dietary habits(P<5e-6)was extracted from the UK Biobank.And linkage disequilibrium(LD r^(2)<0.001,kb=10000),palindromic sequences and incompatible SNP locus information were excluded.Two-sample MR analysis was performed using inverse variance weighting(IVW),MR-Egger,Weighted median,Weighted mode,and simple mode.In the sensitivity analysis,Cochran's Q statistic was used to detect heterogeneity.MR-Egger regression,MR-Pleiotropy residuals and outlier point methods were used to detect pleiotropy.Results The study analyzed the causal relationship between 13 different dietary behaviors and the risk of liver cancer in Japan population.It was found that bread intake increased the risk of liver cancer in Japan population(IVW:OR:1.32,95%CI:1.09-1.60,P:0.004),but no causal associations were obtained between other dietary habits and live cancer.Conclusion Based on the present data,the research concluded that bread intake increased the risk of liver cancer in Japan population,but no causal association between other dietary habits and the occurrence of liver cancer in Japan population could be concluded.