基于主成分分析和响应面法优化夏枯草活性成分超声提取工艺

Optimization in Ultrasonic Extraction Process of Active Components from Prunella vulgaris L.Based on Principal Component Analysis and Response Surface Methodology

建立了夏枯草的指纹图谱,通过主成分分析法进行降维处理,确定了2个主成分,以其综合得分为评价指标,在单因素实验的基础上,采用响应面法优化夏枯草活性成分的超声提取工艺。结果表明,除提取温度外,其它因素均对综合得分有显著影响,影响大小顺序为:超声时间>乙醇体积分数>料液比;确定最佳提取工艺为:超声时间23 min、料液比1∶20(g∶mL)、乙醇体积分数65%。该方法不仅能全面考察夏枯草活性成分的提取效果,同时简化数据,具有一定的实用性,为夏枯草药材的质量控制及进一步开发利用奠定了基础。

We established the fingerprint of Prunella vulgaris L.,and determined two principal components by principal component analysis.Moreover,taking comprehensive score as an evaluation index,we optimized the ultrasonic extraction process of active components from Prunella vulgaris L.by response surface methodologies on the basis of single-factor experiments.The results show that all factors except extraction temperature have significant influence on the comprehensive score,with the order as follows:ultrasonic time>ethanol volume fraction>solid-liquid ratio.The optimal extraction process can be determined as follows:the ultrasonic time of 23 min,the solid-liquid ratio of 1∶20(g∶mL),and the ethanol volume fraction of 65%.This method can not only comprehensively investigate the extraction effect of active components from Prunella vulgaris L.,but also simplify the data.This method has certain practicability,and lays a foundation for the quality control and further development and utilization of Prunella vulgaris L..