火麻籽游离氨基酸提取工艺研究

Study on the extraction process of free amino acid from hemp seeds

以巴马火麻籽为材料,采用超声辅助乙醇提取火麻籽游离氨基酸。以游离氨基酸提取量为指标,在单因素试验的基础上通过响应面法优化提取工艺。结果表明:最优提取工艺为超声时间35min、超声温度46℃、超声功率175W、乙醇体积分数25%,在此条件下获得的火麻籽游离氨基酸提取量为(13.07±1.77)mg/g。

Using Bama hemp seeds as the material,ultrasonic assisted ethanol extraction was used to extract free amino acids from hemp seeds.Using the extraction amount of free amino acids as an indicator,the extraction process was optimized by response surface methodology based on single factor experiment.The results showed that the optimal extraction process was as follows:ultrasound time 35 min,ultrasound temperature 46℃,ultrasound power 175 W,and ethanol volume fraction 25%.Under these conditions,the extraction amount of free amino acids from hemp seeds was(13.07±1.77)mg/g.