软枣猕猴桃花粉多糖提取、表征及体外生物活性研究

Extraction,characterization and in vitro biological activity of polysaccharide from Actinidia arguta Planch.pollen

以软枣猕猴桃花粉为原料,采用超声辅助提取软枣猕猴桃花粉多糖(AAPP)。以AAPP得率为指标,在单因素试验的基础上通过响应面法优化提取工艺,并分析其结构及体外生物活性。结果表明:AAPP最佳提取工艺为超声时间4.10h、超声温度68.0℃、超声功率311W,在此条件下AAPP得率为1.96%±0.12%。AAPP-11总糖含量为89.44%,相对分子质量为15252,由11种单糖构成,其中以物质的量比为21.53:12.77:12.25的葡萄糖(Glc)、鼠李糖(Rham)和葡萄糖醛酸(GlcA)为主。AAPP-11中不含核酸,且蛋白质含量较低,是1种含有β-糖苷键构型及呋喃环构型的多糖,具有抗氧化活性和较强的降血糖活性。

Using Actinidia arguta Planch.pollenas raw material,ultrasonic-assisted extraction of Actinidia arguta Planch.pollen polysaccharides(AAPP)was used.Based on the yield of AAPP as an indicator,the extraction process was optimized using response surface methodology on the basis of single factor experiments,and its structure and in vitro biological activity were analyzed.The results showed that the optimal extraction process for AAPP were as follows:ultrasound time 4.10 h,ultrasound temperature 68.0℃,and ultrasound power 311 W.Under these conditions,the yield of AAPP was 1.96%±0.12%.AAPP-11 has a total sugar content of 89.44%,and a relative molecular weight of 15252.It was composed of 11 monosaccharides,with glucose(Glc),rhamnose(Rham),and glucuronic acid(GlcA)in a molar ratio of 21.53:12.77:12.25.AAPP-11 contains no nucleic acid and low protein content,and itwas a polysaccharide containingβ-glucoside bond configuration and furan ring configuration,whichhas antioxidant activity and strong hypoglycemic activity.