谷子γ-氨基丁酸转运蛋白基因的生物学及表达特征分析

Biological and expression characteristics analysis ofγ-aminobutyric acid transporter gene in Setaria italica

[目的]γ⁃氨基丁酸是一种可以调节植物抗逆性的重要氨基酸,参与植物的生长、发育及应对各种胁迫,而γ⁃氨基丁酸转运蛋白(GAT)可以将其转运到特定部位从而发挥作用。对谷子GAT基因进行研究可为后续探究谷子的抗逆机制发挥重要作用。[方法]本研究对谷子GAT进行了系统的生物信息学分析,并通过实时荧光定量PCR分析谷子GAT在胁迫条件下的表达情况;此外,对拟南芥野生型和SALK_201083C(谷子GAT的拟南芥同源基因T⁃DNA插入突变体)进行抗旱性研究。[结果]研究表明,谷子GAT具有明显的组织表达特异性,能够响应逆境胁迫,并参与调节谷子干旱胁迫应答的机制。拟南芥SALK_201083C的抗旱性显著减弱。[结论]谷子GAT能够参与植物的逆境调节。研究结果对今后进一步研究GAT的功能提供一定理论依据。

[Objective]γ-aminobutyric acid is an important amino acid that can regulate plant stress resistance,participating in plant growth,development,and response to various stresses,whileγ-aminobutyric acid transporter(GAT)can transport it to specific sites to exert its effects.Studying the GAT gene in Setaria italica can play an important role in exploring the stress re⁃sistance mechanism of foxtail millet in the future.[Methods]In this study,a systematic bioinformatics analysis of GAT gene in S.italica,Si7g07810,was performed,and the expression of Si7g07810 under stress conditions was analyzed by real-time fluo⁃rescent quantitative PCR;In addition,the drought resistance of T-DNA insertion mutants of Arabidopsis wild-type and At5g41800(Arabidopsis homologous gene of Si7g07810)was studied.[Results]Research showed that GAT gene in S.italica had significant cell expression specificity,could respond to stress and participated in regulating the mechanism of millet drought stress response.The drought resistance of Arabidopsis At5g41800T-DNA insertion mutant was significantly reduced.[Conclu⁃sion]GAT gene in S.italica can participate in the regulation of plant stress.The research results provide a theoretical basis for further studying the function of GAT gene in the future.