苦瓜DNA分子标记研究进展

Research progress on DNA molecular marker of bitter melon

我国苦瓜品种资源较丰富,南北各地均有分布与栽培,多见于南方各省市。苦瓜DNA分子标记研究开展相对较晚,标记类型应用较少,分子标记辅助育种成果鲜有报道。总结了RAPD、AFLP、SSR、ISSR、SRAP及SNP等第二代和第三代DNA分子标记在苦瓜品种和种子纯度鉴定、亲缘关系分析、遗传图谱构建、QTL定位等方面的研究进展,以期为苦瓜遗传育种等研究提供参考依据。同时在苦瓜现有研究基础上进行展望,认为应加快挖掘功能性基因,开发功能性分子标记及全基因组SNP芯片,这对后续研究具有重大意义。

China is rich in variety resources of bitter melon.This vegetable is distributed all over the country,mostly in the southern part.The research on DNA molecular markers of bitter melon started relatively late.The types of DNA marker applied in bitter melon are few,and the application of marker-assisted breeding are rarely reported.Here we summarized the research progress and applications of the second and the third generation of DNA makers,such as RAPD,AFLP,SSR,ISSR,SRAP and SNP,in variety and seed purity identification,genetic relationship analysis,genetic map construction,QTL mapping in bitter melon.Based on these information,it is expected that the exploration of functional genes,development of functional molecular markers and whole-genome SNP chips should be accelerated.