黄瓜新品种京研冬美9号的SSR纯度鉴定

SSR purity identification of cucumber variety Jingyan Dongmei No.9

种子纯度鉴定是保证种子质量的关键环节。田间小区种植鉴定法,周期长、费用高,无法满足种子贸易中快速鉴定的要求,且受环境影响大,结果不够准确。DNA电泳鉴定技术为种子的纯度鉴定提供了一种更为快速、高效的方法。笔者采用72对SSR引物对黄瓜品种京研冬美9号及其亲本进行多态性筛选。结果表明,12对引物在亲本间具有多态性,并都表现出共显性,在杂交后代中表现为清晰、稳定的父母本互补的带型,特异性强,可以用来区别其中混杂的母本、父本及其他品种的种子。这12对SSR引物作为京研冬美9号及其亲本的核心引物,构成的京研冬美9号的SSR指纹图谱,为京研冬美9号杂交种的亲本提纯、纯度检测及真实性鉴定等工作提供了有效的技术支持。其中,2对引物SSR17922和SSR02118特异性强且谱带清晰,利用这2对引物对京研冬美9号黄瓜种子进行检测,鉴定结果与田间鉴定结果一致,被选为鉴定黄瓜京研冬美9号纯度的特异引物。

For increasing the efficiency of application of the variety identification and seed genetic purity on cucumber hybrid seed,ensuring the seed genetic quality,alternative 72 pairs of SSR primers have been applied in the screening the molecular markers on the cucumber Jingyan Dongmei No.9 and their parents.The results showed that 12 pairs of primers complementary to appear polymorphic bands between the parents,clear and stabilized,performing codominance,highly specificity,among hybrids,which can be used to distinct its parents and other varieties.The core SSR primers of Jingyan Dongmei No.9 were composed mainly of these 12 pairs of primers,formed the SSR fingerprint of Jingyan Dongmei No.9,which provided technological information for seeds purity,authenticity of hybrid and parents purifying.Among them,the bands amplified by 8 pairs of primers were highly distinguishable,the particularity was strong,as well as the characteristics of clear bands and the result agreed with field test.