腹腔镜术后防粘连用CS/PCL共混纤维膜的制备与表征

Preparation and characterization of CS/PCL blended fiber membranes for anti-adhesion after laparoscopic surgery

为开发一款生物安全性高、力学性能优异且防粘连作用佳的腹腔镜术后防粘连膜,利用静电纺丝技术,将壳聚糖(CS)和聚己内酯(PCL)按不同质量比共混纺丝,对所得共混纤维膜的表观形貌、力学性能、浸润性及防细胞黏附性进行测试与分析。结果表明:随着CS质量分数的增大,共混纤维膜的可纺性大幅下降,并且CS质量分数超过40%时纤维均匀性变差,纤维膜的结晶度先增大后减小,拉伸强度、弹性模量、弯曲刚度增大,断裂伸长率减小;相比纯PCL纤维膜,CS/PCL共混纤维膜的浸润性得到明显改善,对成纤维细胞的黏附能力减弱,防粘连性能优异。综合考量材料成本、可纺性、力学性能及防粘连效果,认为CS质量分数为30%的共混纤维膜在腹腔镜术后防粘连领域的潜在应用价值最大。

To develop a post-laparoscopic anti-adhesion film with high biosafety,excellent mechanical properties and good anti-adhesion effect,chitosan(CS)and polycaprolactone(PCL)were blended with different mass ratios by electrospinning technology.The apparent morphology,mechanical properties,wettability and anti-adhesion of the obtained fiber membranes were tested and analyzed.The results show that with the increase of CS mass fraction,the spinnability decreases greatly.When CS mass fraction exceeds 40%,the fiber uniformity becomes worse.The crystallinity of the blended fiber membranes first increases and then decreases,the tensile strength,elastic modulus and bending stiffness increase,and the elongation at break decreases.Compared with pure PCL electrospun membrane,the wettability of the blended sample is significantly improved,the adhesion to fibroblasts is weakened,and the anti-adhesion effect is excellent.Based on Considered the raw material cost,spinnability,mechanical properties and anti-adhesion effect,the blended fiber membrane with 30%CS mass fraction has the most potential application value in the field of anti-adhesion after laparoscopic surgery.