截形叶螨雌成螨应对高温胁迫的转录组分析

Transcriptome Analysis of Response to Heat Stress of Female Adult Mites of Tetranychus truncatus

为明确截形叶螨Tetranychus truncatus雌成螨应对热胁迫的差异表达基因,采用Illumina HiSeq 4000进行转录组测序。结果表明,截形叶螨雌成螨热胁迫后共检测到17577个差异基因,其中12831个上调,4746个下调。GO功能富集发现,雌成螨热胁迫后的差异基因主要富集于细胞过程、催化活性和细胞,KEGG通路富集分析发现,差异基因主要富集在代谢途径和溶酶体等。从转录组测序数据中筛选出与高温存在关联的抗氧化酶和热激蛋白等基因。采用RT-qPCR技术检测这些基因在截形叶螨雌成螨上的表达特征,发现17条基因的上下调趋势与转录组测序结果一致。这为后续深入研究截形叶螨与热胁迫相关基因的功能奠定了基础。

Tetranychus truncatus is a key pest on many crops such as maize,vegetables,which always causes asignificant economic losses even in hot environment.In order to identify the differentially expressed genes(DEGs)of the female adult mites of T.truncatus under heat stress,transcriptome sequencing of T.truncatus was conducted by using Illumina HiSeq 4000.The results showed that a total of 17577 DEGs were detected in female adult mites of T.truncatus,among which 12831 were up-regulated and 4746 were down-regulated.It was also found that the DEGs of female adult mites of T.truncatus were enriched the cellular processes,catalytic activity and cellby GO functional enrichment analysis,and the DEGs were enriched in metabolic pathways and lysosomes by KEGG pathway enrichment.Moreover,antioxidant enzymes and heat shock protein(Hsp)related to heat stress were screened from the transcriptome database as well.Then the expression characteristics in female adult of T.truncatus about the previous genes were tested by RT-qPCR technique.We found that the up-regulation trend of 17 genes are consistent with above transcriptome sequencing results.This study provides a basic foundation for the further discussion on the function of genes related to heat stress of T.truncatus.