超声促进葡聚糖-酪蛋白磷酸肽接枝及其递送钙的应用

Ultrasound-Promoted Dextran-Casein Phosphopeptide Grafting and Its Application in Calcium Delivery

为了进一步完善蛋白质与多糖共聚物作为钙递送体系的应用,本研究利用超声法促进葡聚糖与酪蛋白磷酸肽(casein phosphopeptide,CPP)间的接枝,并构建葡聚糖-酪蛋白磷酸肽-钙离子(葡聚糖-CPP-Ca^(2+))递送体系,以傅里叶红外光谱(FTIR)、扫描电子显微镜(SEM)等方法表征其结构,探讨其在模拟胃肠道消化中的缓释性能.结果表明:超声处理增加了CPP与葡聚糖结合点位,与水浴处理组相比,在接枝率为50.00%时共聚物反应时间缩短2.5 h,且在反应4 h后,250W超声处理组的接枝率比水浴处理组提高19.67%,葡聚糖-CPP-Ca^(2+)在胃液和小肠液消化阶段过程中钙离子的释放率分别为64.48%和89.62%,表明缓释效果良好.本研究结果为促进葡聚糖-CPP接枝反应与递送钙的应用提供了基础.

In order to further improve the application of protein and polysaccharide copolymers as calcium delivery systems,in this study we used ultrasound to promote the grafting process between dextran and casein phosphopeptide(CPP)and construct dextran-casein phosphopeptide-calcium ion(referred to as dextran-CPP-Ca^(2+))nutrient delivery system.The system was further characterized by Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM)and other methods with an intended purpose to explore its sustained-release properties in simulated gastrointestinal digestion.The results showed that ultrasonic treatment increased the number of binding sites between CPP and dextran.Compared with the water bath group,the reaction time of the copolymer was reduced by 2.5 h when the grafting rate was about 50.00%.Moreover,after 4 h of reaction,the grafting rate of the 250W ultrasonic treatment group was increased by 19.67%compared with the water bath group,and the calcium ion release rates of dextran-CPP-Ca^(2+)in the artificial gastric juice and artificial small intestinal juice digestion stage were 64.48%and 89.62%,respectively,with good sustained release effect.Therefore,the research results has provided a basis for promoting the application of dextran-CPP grafting reaction and delivery system.