硫酸化木聚糖的制备及其体外益生菌增殖作用研究

Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics

本研究旨在确定硫酸化木聚糖对益生菌生长的影响,根据氨基磺酸-N,N-二甲基甲酰胺法对木聚糖(xylan,Xyl)进行化学改性,产生硫酸化木聚糖(sulfated xylan,SXY)。采用傅里叶红外光谱、扫描电子显微镜和刚果红实验对SXY进行结构表征,利用德氏乳杆菌保加利亚亚种GIM1.155、植物乳杆菌GIM1.191、短乳杆菌GIM1.773和嗜热链球菌GIM1.540共4种肠道益生菌对其体外增殖作用进行研究。结果表明:红外光谱显示SXY在1243、1052和894 cm^(-1)处分别出现由S=O伸缩振动、C—O拉伸和C—O—SO_(3)基团对称C—O—S伸缩振动引起的特征吸收峰。扫描电镜显示得到表面结构平滑度增加的SXY。刚果红实验显示SXY具有三螺旋结构。采用BaCl_(2)-明胶比浊法测得SXY取代度为0.341。体外实验结果显示SXY对益生菌体外增殖最佳浓度为2.0%,培养10 h后观察到快速生长和增殖增加。所获结果表明,SXY对益生菌的生长有促进作用,是维持肠道健康的益生元替代物。

This study aimed to determine the effect of sulfated xylans on the growth of probiotics.Xylan(Xyl)was chemically modified using the sulfamic acid-N,N-dimethylformamide method to produce sulfated xylan(SXY).The SXY was characterized by Fourier infrared spectroscopy(FT-IR),scanning electron microscope(SEM)and Congo red experiment.The Xyl and SXY were tested for their in vitro prebiotic effects using four strains of intestinal microflora,including Lactobacillus delbrueckii subsp.bulgaricus(GIM1.155),L.plantarum(GIM1.191),L.brevis(GIM1.773),and Streptococcus thermophilus(GIM1.540).From the recorded infrared spectra,vibrational bands for SXY were observed at 894,1052 and 1243 cm^(−1),they could be assigned to the C—O—S vibration of a C—O—SO_(3),C—O stretched and S=O stretching vibrations.The results of scanning electron microscopy showed that the surface structure of SXY increased smoothness.Congo red experiment revealed that SXY had a triple helix structure.The substitution degree of SXY measured using a BaCl_(2)-gelatin turbidimeter was 0.341.In vitro experiment showed that the optimum concentration of SXY for the in vitro probiotic growth was 2.0%,rapid growth and increased proliferation were observed after 10 h of culture.The results obtained suggest that SXY could promote probiotic growth.It is an alternate source of prebiotics for maintaining gut health.