响应面法优化铁皮石斛软糖配方及其抗氧化活性分析

Optimization of Dendrobium offcinale Fondant Formulation and Its Antioxidant Activity Determination by Response Surface Methodology

以铁皮石斛为主要原料,以赤藓糖醇、柠檬酸、明胶为辅料来制作铁皮石斛软糖。以感官评定为指标,采用单因素实验和响应面试验研究铁皮石斛软糖的最佳制作工艺及配方。再运用最优铁皮石斛软糖对DPPH自由基、超氧阴离子自由基清除实验及其还原能力的测定来说明软糖的体外抗氧化活性;同时测定铁皮石斛软糖的质构特性。结果表明:铁皮石斛添加量为92.90%,明胶添加量18.30%,柠檬酸添加量0.14%,赤藓糖醇添加量29.20%为最佳,此时感官评分为83.50分。根据最优配方制得的铁皮石斛软糖,分析得出其还原糖、多酚含量分别为30.03 g/100 g、0.85 mg/g。体外抗氧化分析结果显示,铁皮石斛软糖的体外抗氧化活性与VC相近,铁皮石斛软糖对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的半数有效浓度(IC_(50))为0.50 mg/mL、清除超氧阴离子自由基的IC_(50)值为0.13 mg/mL,可证明铁皮石斛软糖具有良好的抗氧化能力。质构分析结果得出铁皮石斛软糖硬度在4600~4800 g之间,软硬适宜;铁皮石斛软糖咀嚼性在4200~4300 g范围内,接受度较高。

Dendrobium offcinale was used as the main raw material,and erythritol,citric acid and gelatin were used as auxiliary ingredients to make Dendrobium offcinale fondant.Using sensory evaluation as the index,single-factor experiments and response surface experiments were used to study the optimal production process and formulation of Dendrobium offcinale fondant.Then,the in vitro antioxidant activity of the fondant was illustrated by the determination of the scavenging of DPPH radicals and superoxide anion radicals and their reducing ability of the optimal Dendrobium offcinale fondant,meanwhile,the textural properties of Dendrobium offcinale fondant were determined.The results showed that the addition of 92.90%of Dendrobium offcinale,18.30%of gelatin,0.14%of citric acid and 29.20%of erythritol was the best,and the sensory score was 83.50 at that time.The reducing sugar and polyphenol contents of Dendrobium offcinale fondant made according to the optimal formulation were analyzed as 30.03 g/100 g and 0.85 mg/g.Respectively,the results of in vitro antioxidant analysis showed that the in vitro antioxidant activity of Dendrobium offcinale fondant was similar to that of VC,and the half effective concentration(IC_(50))of Dendrobium offcinale fondant against 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical was 0.50 mg/mL and the IC_(50) value for the scavenging superoxide anion radicals was 0.13 mg/mL,which could prove that Dendrobium offcinale fondant had good antioxidant ability.The results of texture analysis concluded that the hardness of Dendrobium offcinale fondant ranged from 4600 to 4800 g,with suitable softness and hardness, the chewiness of Dendrobium offcinale fondant ranged from 4200 to 4300 g, and the acceptability was higher.