基于秀丽隐杆线虫模型的樱桃乙醇提取物抗衰老作用研究

Study on the Anti-aging Effect of Cherry Ethanol Extract Based on Caenorhabditis elegans Model

本文以胶东地区常见的两种樱桃品种—红灯和黄蜜为研究对象,探究并比较了樱桃的抗衰老作用,为樱桃的进一步开发利用提供依据。对两种樱桃的果肉和果核分别进行醇溶提取并初步明确成分,以DPPH自由基和羟自由基清除能力为指标评价四种成分的抗氧化活性并探究其抑制β淀粉样蛋白(Aβ)聚集的能力。以秀丽隐杆线虫为模型,测定四种成分对其寿命、运动能力、生殖能力、瘫痪缓解能力的作用效果和抑制Aβ沉积情况。结果表明:两种樱桃果肉的抗氧化活性突出,样品浓度在1 mg/mL时,黄蜜和红灯的DPPH自由基清除率分别为80.88%和83.19%,羟自由基清除率分别为88.19%和92.85%。果核相对较低,黄蜜和红灯的DPPH自由基清除率分别为20.76%和44.93%,羟自由基清除率分别为28.65%和38.75%。四种样品均对Aβ表现出抑制作用,且在线虫实验中,与空白组相比,四种样品组的线虫寿命能够延长2~4 d,增强其运动能力,对生殖能力无损害且降低线虫瘫痪率,减少Aβ沉积。综上,四种样品能够通过清除自由基,抑制Aβ的产生从而产生一定的抗衰老效果,具有潜在的应用价值。

In this paper,the anti-aging effects of two varieties of cherry,Red Light and Yellow Honey in Jiaodong region were investigated and compared,for further development and utilization of cherries.The components of pulp and kernels in the two kinds of cherries were extracted by ethanol and the antioxidant activity was evaluated by the scavenging ability of DPPH and hydroxyl radicals,the ability to inhibit the aggregation ofβ-amyloid protein(Aβ)was investigated.The effects of four components on the life span,locomotor ability,reproductive ability,paralysis relief ability and the inhibition of Aβdeposition of Caenorhabditis elegans were studied.The results showed that antioxidant activity of two cherry pulp was outstanding,with DPPH radical scavenging rates of 80.88%and 83.19%and hydroxyl radical scavenging rates of 88.19%and 92.85%for Yellow Honey and Red Light,respectively,when the sample concentration was 1 mg/mL.While the antioxidant activity of fruit kernels was relatively low,with DPPH and hydroxyl radical scavenging rates of Yellow Honey and Red Light were 20.76%and 44.93%,28.65%and 38.75%,respectively.Compared with the blank group,the life span of Caenorhabditis elegans in the four groups was prolonged by 2~4 days and their locomotor ability was enhanced,and it did not damage the reproduction ability and reduced the paralysis rate of Caenorhabditis elegans and Aβdeposition.In conclusion,the four samples were able to produce certain anti-aging effects by scavenging free radicals and inhibiting the production of Aβ,which have potential applications.