神经元表达发育下调基因9诊断胰腺癌及其对患者预后的预测价值

Value of NEDD9 for Diagnosis and Prediction of Prognosis in Pancreatic Cancer

【目的】探讨神经元表达发育下调基因9(NEDD9)诊断胰腺癌及其预测患者预后的价值。【方法】80例在本院首次就诊并经手术中快速病理活检证实的胰腺癌患者,收集患者胰腺癌组织及癌旁正常组织,采用免疫组化及qPCR技术检测各组织NEDD9的表达情况,分析NEDD9与胰腺癌患者临床特征的关系,探讨NDEE9对胰腺癌患者临床预后的影响。【结果】胰腺癌组织中的NEDD9高表达率为71.25%,显著高于癌旁组织的51.25%(P<0.05),其NEDD9 mRNA水平(0.92±0.17)也显著高于癌旁正常组织(0.33±0.09),差异有统计学意义P<0.05);低表达NEDD9患者的临床分期(Ⅰ~Ⅱ期)、分化程度(高分化)显著优于高表达NEDD9患者,同时淋巴结转移率显著低于高表达患者(均P<0.05);随访结果显示,发生预后不良的患者NEDD9表达水平(1.21±0.31)显著高于预后良好组(0.73±0.25)(P<0.05);二元Logistic回归分析显示,高表达NEDD9是胰腺癌患者预后不佳的独立危险因素,其优势比为2.18(1.16~4.01)。【结论】NEDD9在胰腺癌组织中呈显著高表达,且与患者临床分期及病例分级有相关性,NEDD9高表达是胰腺癌患者预后不佳的独立危险因素。

【Objective】To investigate the value of neuronal expression developmentally downregulated gene 9(NEDD9)for diagnosis and prediction of prognosis in pancreatic cancer.【Methods】A total of 80 patients with pancreatic cancer,who were treated for the first time in our hospital and confirmed by rapid pathological biopsy,were enrolled in the study.The pancreatic cancer tissues and normal tissues adjacent to the cancer were collected.The expression of NEDD9 in pancreatic cancer tissues and normal tissues was detected by both immunohistochemistry and qPCR.The relationship between NEDD9 and clinical features of pancreatic cancer was analyzed.And the effect of NDEE9 on clinical prognosis of patients with pancreatic cancer was discussed.【Results】Compared to normal tissues,the expression rate of NEDD9 in pancreatic cancer tissues was significantly higher(71.25%vs 51.25%,P<0.05);And the level of NEDD9 mRNA was also significantly higher than that of normal tissue(0.92±0.17)vs(0.33±0.09),P<0.05).The clinical stage(stageⅠ-Ⅱ)and degree of differentiation(high differentiation)in patients with low expression of NEDD9 were significantly better than those who had higher expression level of NEDD9.Follow-up results showed that the expression level of NEDD9 in patients with poor prognosis(1.21±0.31)was significantly higher than that in the group with good prognosis(0.73±0.25)(P<0.05).Binary logistic regression analysis found that high expression of NEDD9 was an independent risk factor for negative prognosis of pancreatic cancer,and its odds ratio was 2.18(1.16-4.01).【Conclusion】NEDD9 was highly expressed in pancreatic cancer tissues and it was associated with clinical stage and pancreatic cancer grading.The high expression of NEDD9 was an independent risk factor for negative prognosis in patients with pancreatic cancer.