黑木耳菌株的菌丝生长速度对胞外酶活性的影响

Effect of mycelial growth rate of Auricularia heimuer strains on extracellular enzyme activity

以12个菌株为研究对象,分别测定漆酶、纤维素酶和木聚糖酶3种胞外酶活性,并结合菌丝日均生长速度分析两者的关系,为黑木耳优质菌株筛选和选育提供参考依据。结果表明:供试菌株依据长速快慢通过显著性差异(P<0.005)分析可分为3组。3种胞外酶在多数菌株间存在显著差异,漆酶和纤维素酶活性与菌丝长速存在正相关关系,而木聚糖酶则无明显相关性;说明通过菌丝长速快慢可以初步推断漆酶和纤维素酶活性高低,但无法判断木聚糖酶活性情况。

Taking 12 strains of Auricularia heimuer as the research objects,the activities of three extracellular enzymes(laccase,cellulase and xylanase)were determined respectively,and the relationship between the extracellular enzyme activities and the average daily growth rate of the mycelia was analyzed.The results showed that the tested strains could be divided into three groups according to the significant difference(P<0.005)in mycelial growth rate.There were significant differences in the three extracellular enzymes among most strains.The activities of laccase and cellulase were positively correlated with the mycelial growth rate,while xylanase had no significant correlation.In conclusion,the activities of laccase and cellulase could be inferred by the mycelial growth rate,but the activity of xylanase could not be judged.