黔产追风伞总黄酮含量测定

Determination of total flavonoids in Lysimachia paridiformis from Guizhou

目的:优选追风伞中总黄酮的提取工艺,建立追风伞总黄酮含量测定方法,测定贵州不同产地追风伞药材中总黄酮的含量。方法:以芦丁为对照品,采用紫外-分光光度计法,测定不同产地追风伞药材中总黄酮的含量。结果:根据单因素试验确定了总黄酮的最佳提取工艺,以50%乙醇作为提取溶剂,回流提取30 min,在500 nm波长下所得的总黄酮含量最高。测得15批追风伞中总黄酮含量在2.606%~5.670%之间,平均值为4.389%。结论:优选的提取工艺稳定可行,该方法操作简单、重复性好,可为追风伞药材质量标准的制定提供参考。

The extraction process of Lysimachia paridiformis was optimized,and a method for the determination of the total flavonoids in Lysimachia paridiformis was establishedto determine the total flavonoids in medicinal Lysimachia paridiformis from different producing areas in Guizhou.Using rutin as the reference material,the total flavonoids content in Lysimachia paridiformis was determined by ultraviolet spectrophotometry.The optimum extraction process of total flavonoids was determined by single factor tests as follows:the extraction solvent was 50%ethanol,and the reflux extraction time was 30 min.The maximum content of total flavonoids was obtained at 500 nm wavelength.The total flavonoids in the 15 batches of Lysimachia paridiformis was 2.606%to 5.670%,with an average of 4.389%.The optimized extraction process is stable and feasible,and the method is simple to operate and has good repeatability,which can provide reference for the formulation of the quality standard of medicinal Lysimachia paridiformis.