摘要
精准的结构和功能成像对于泌尿系统疾病的诊断和预后至关重要。近红外二区(second near-infrared,NIR-Ⅱ;1000~1700 nm)荧光生物成像相较于传统的荧光生物成像具有更高的空间分辨率、更深的穿透力和更好的信噪比,但其临床适用性有限。本文首次报道了基于临床可用且经肾脏排泄的荧光染料亚甲蓝(methylene blue,MB)NIR-Ⅱ活体泌尿系统荧光成像,该方法不仅可以实现清晰的有创/无创尿路造影,而且可以有效且无创地检测肾功能。以上研究结果表明MB辅助的NIR-Ⅱ荧光成像在临床及基础研究中的泌尿系统结构和功能成像中具有巨大前景。
Accurate structural and functional imaging is vital for the diagnosis and prognosis of urinary system diseases.Fluorescence bioimaging in the second near-infrared spectral region(NIR-II,1000-1700 nm)has shown advantages of higher spatial resolution,deeper penetration,and finer signal-to-background ratio(SBR)compared to the conventional fluorescence imaging methods but limited to its clinical inapplicability.Herein,we first report in vivo NIR-II fluorescence imaging of the urinary system enabled by a clinically approved and renal excretable dye methylene blue(MB),which cannot only achieve clear invasive/non-invasive urography but also noninvasively detect renal function efficiently.These results demonstrate that MB assisted NIR-II fluorescence imaging holds a great promise for structural and functional imaging of the urinary system both clinically and preclinically.
作者
薛丁玮
吴迪
陆泽毅
Jochen Neuhaus
阿卜杜热合曼·则比布拉
冯哲
成晟
周静
钱骏
李恭会
Dingwei Xue;Di Wu;Zeyi Lu;Jochen Neuhaus;Abudureheman Zebibula;Zhe Feng;Sheng Cheng;Jing Zhou;Jun Qian;Gonghui Li(Department of Urology,Sir Run Run Shaw Hospital,School of Medicine,Zhejiang University,Hangzhou 310016,China;Department of General Surgery,Sir Run Run Shaw Hospital,School of Medicine,Zhejiang University,Hangzhou 310016,China;Department of Urology,University of Leipzig,Leipzig 04103,Germany;State Key Laboratory of Modern Optical Instrumentation,Centre for Optical and Electromagnetic Research,College of Optical Science and Engineering,Zhejiang University,Hangzhou 310058,China)
基金
the National Natural Science Foundation of China(81672520,81870484,61975172,and 82001874,used for procurement of materials and labor cost)
Science and Technology Planning Project of Zhejiang(2019C03089,used for procurement of materials)
Zhejiang Provincial Natural Science Foundation of China(LR17F050001,used for procurement of materials).
