脂肪酶催化醇解制备富含n-3 PUFAs的甘油酯

Preparation of Glycerides Rich in n-3 PUFAs by Lipase-catalyzed Alcoholysis

n-3 PUFAs具有多种生理活性。以鱼油为原料,采用脂肪酶催化醇解法对其中的n-3 PUFAs进行富集,得到富含n-3 PUFAs的甘油酯。结果表明:来自米曲霉的脂肪酶Eversa Transform 2.0(ET 2.0)对n-3 PUFAs表现出良好的醇解特异性。通过单因素实验确定最佳工艺条件为:甲醇体积分数40%,m_(甲醇)∶m_(鱼油)=1.6∶1,温度35℃,脂肪酶添加量4%,反应时间2 h。在此条件下,鱼油甘油酯中n-3 PUFAs的含量从39.88%上升至67.87%,EPA和DHA的含量从36.61%上升至65.22%,n-3 PUFAs的得率为56.33%。脂肪酶在最佳反应条件下对不同底物的富集效果是:金枪鱼油甘油酯中n-3 PUFAs的含量从38.81%上升到61.35%,藻油甘油酯中n-3 PUFAs的含量从45.96%上升到64.09%。上述方法获得的富含n-3 PUFAs甘油酯在食品和制药行业有很大的应用潜力。

n-3 PUFAs have various physiological activities,but their contents in natural oils are usually low.This study investigated the preparation of glycerides rich in n-3 PUFAs by Eversa Transform 2.0(ET 2.0)-catalyzed alcoholysis of fish oil.The results showed that the ET 2.0 from Aspergillus oryzae could effectively identify n-3 PUFAs during alcoholysis.Saturated fatty acids and monounsaturated fatty acids were released from fish oil by ET 2.0-catalyzed alcoholysis,causing the production of n-3 PUFA-enriched glycerides.Under optimal conditions(1.6∶1 of mass ratio of methanol to algal oil,40%methanol aqueous solution,4%ET 2.0 dosage,35℃,2 h),the n-3 PUFA content in glyceride increased from the initial 39.88%to 67.87%,the content of EPA and DHA increased from 36.61%to 65.22%,and the yield of n-3 PUFAs was 56.33%.When tuna oil and algal oil were used as substrate,the content of n-3 PUFAs in tuna oil glycerides increased from 38.81%to 61.35%,and the content of n-3 PUFAs in algae oil glycerides increased from 45.96%rose to 64.09%.The n-3 PUFAs-enriched glycerides obtained by the above method have great application potential in the food and pharmaceutical industries.