High-Resolution Multiscale Imaging Enabled by Hybrid Open-Top Light-Sheet Microscopy

1.Introduction Light-sheet fluorescence microscopy(LSFM),since it was first coined the term selective-plane-illumination microscopy(SPIM)by Huisken et al.[1],has been developed for nearly two decades,needless to mention a landmark development of digitally scanned light-sheet microscopy(DSLM)innovated by Keller et al.[2].