LC-MS/MS测定菊花不同加工方式下8种农药的加工因子

Determination of Processing Factors of 8 Pesticides in Chrysanthemi Flos Extracted under Different Conditions by LC/MS/MS

目的:通过考察菊花中8种易检出农药在不同加工方式下的加工因子,为菊花服用安全性评估提供参考依据。方法:使用菊花农药残留阳性样品,模拟菊花常用的加工方法(水煎煮、冲泡、70%乙醇提取),并采用液相色谱-质谱法测定加工过程中菊花各阶段的农药残留量,计算农药转移率和加工因子。结果:采用水煎煮和冲泡法,8种农药的转移率差异较大,呈现与农药的极性正相关的变化规律;而采用70%乙醇提取,8种农药转移至终产品中的残留量均较高。结论:通过对不同加工方式下菊花农药加工因子进行研究,为菊花中农药残留限度的制定提供参考依据,并为科学评估菊花中农药残留风险提供数据支持。

Objective:To calculate the processing factors of 8 pesticides in Chrysanthemi Flos extracted under different methods and provide reference for the safety assessment of direct oral administration.Methods:Chrysanthemi Flos samples with pesticide residues were extracted with the commonly used Chinese herbal medicine processing methods,including water brewing,water decocting,and 70%ethanol extracting.The pesticide residues were determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS),and then the pesticide transfer rates and processing factors were calculated.Results:The transfer rates of the 8 pesticides were significantly different between the samples processed by decocting and brewing,showing a positive correlation with the polarity of the pesticides.The transfer rates of 8 pesticides residues in the final products extracted with 70%ethanol were high.Conclusion:According to the difference in pesticide transfer rate of Chrysanthemi Flos samples extracted under different conditions,the processing factors can be calculated as a reference for determining the pesticide residue limit of Chrysanthemi Flos and provide data support for obtaining more accurate results in the risk assessment of pesticide residues.