基于UPLC特征图谱和多成分定量的不同产地绵茵陈药材质量评价

Quality Evaluation of Artemisia scoparia from Different Producing Areas Based on UPLC Characteristic Chromatogram and Multi-component Determination

目的:建立绵茵陈药材超高效液相色谱法(UPLC)特征图谱和6个酚酸类成分含量测定方法,为全面评价其质量提供依据。方法:采用UPLC建立绵茵陈药材特征图谱,对不同产地药材进行相似度评价和聚类分析;同时对6个酚酸类成分含量进行测定。结果:建立了绵茵陈UPLC特征图谱,确定7个共有特征峰,指认了其中6个特征峰,19批绵茵陈药材相似度均大于0.95;聚类分析可将不同产地的药材分为两类,表明不同产地药材存在一定差异;所建立的特征图谱方法可有效地区分不同采收期的花茵陈和绵茵陈药材;含量测定结果显示,河南和甘肃产地药材绿原酸含量和6个酚酸类成分总含量较高且稳定。结论:该方法可以有效评价不同产区绵茵陈药材的质量差异性,为其质量控制和药材选购提供参考。

Objective:To establish the ultra-high performance liquid chromatography(UPLC)characteristic chromatogram and the determination method of six phenolic acid components of Artemisia scoparia to provide a basis for the comprehensive quality evaluation.Methods:UPLC method was used to establish the specific chromatogram of A.scoparia.A.scoparia samples from different producing areas were subjected to similarity evaluation and cluster analysis.At the same time,the content of six phenolic acids was determined.Results:The UPLC characteristic chromatogram of A.scoparia was established.Seven common characteristic peaks were determined and six characteristic peaks were identified.The similarity of 19 batches of A.scoparia was higher than 0.95.A.scoparia samples from different producing areas were classified into three categories by cluster analysis,indicating that there were certain differences in medicinal materials from different producing areas.The established characteristic chromatogram could effectively distinguish A.scoparia medicinal materials from different harvest periods.The results of content determination showed that the content of chlorogenic acid and the total content of six phenolic acids in the medicinal materials from Henan and Gansu was relatively high and stable.Conclusion:The method can effectively analyze the differences in A.scoparia from different producing areas and provide references for the quality control and selection of A.scoparia.