米碎花的酚苷类化合物及其鼻咽癌细胞毒活性

Phenolic Glycosides from Eurya chinensis and Their Cytotoxic Activities Against Nasopharyngeal Carcinoma Cells

为了解米碎花(Eurya chinensis)的化学成分及其生物活性,运用多种色谱技术从其乙醇提取物分离得到11个化合物,并对化合物进行体外抗鼻咽癌细胞增殖活性评价。经波谱数据分析,分别为异落新妇苷(1)、3,5,7-三羟基色原酮-3-O-α-L-鼠李糖苷(2)、1-O-反式-桂皮酰基-β-D-葡萄糖(3)、1-O-(4-羟基苯乙基)-6-O-反式-桂皮酰基-β-D-葡萄糖(4)、eutigoside D(5)、1-O-(3,4-二羟基苯乙基)-6-O-反式-香豆酰基-β-D-葡萄糖(6)、eutigoside A(7)、1-O-(4-羟基苯乙基)-6-O-反式-咖啡酰基-β-D-葡萄糖(8)、grayanoside A(9)、1-O-(4-羟基苯乙基)-6-O-(4-羟基苯甲酰基)-β-D-葡萄糖(10)、3-O-β-D-葡萄糖基-4-羟基-苄基苯甲酸酯(11)。其中,化合物4为首次从天然来源获得,化合物2~4和8~11均为首次从该属植物中分离得到。MTT法表明,化合物10具有中等抑制5-8F细胞增殖活性。

To clarify the chemical constituents and their biological activities of Eurya chinensis,eleven compounds were isolated from its MeOH extract by various column chromatography methods,and the anti-proliferative activity of compounds against human nasopharyngeal carcinoma cells(5-8F)were evaluated.Basis on spectral data,their structures were identified as isoastinlbin(1),3,5,7-trihydroxychromone-3-O-α-L-arabino-pyranoside(2),1-O-trans-cinnamoyl-β-D-glucopyranose(3),2-(4-hydroxyphenyl)ethyl-6-O-(E)-cinnamoyl-β-D-glucopyranoside(4),eutigoside D(5),6-O-coumaroyl-1-O-[2-(3,4-dihydroxyphenyl)ethyl]-β-D-glucopyranoside(6),eutigoside A(7),2-(4-hydroxypheny)ethyl-[6-O-(E)-caffeoyl]-β-D-glucopyranoside(8),grayanoside A(9),4-hydroxyphenylethyl-1-O-β-D-[6′-O-(4-hydroxybenzoyl)]-glucopyranoside(10),benzyl-4′-hydroxybenzoyl-3′-O-β-D-glucopyranoside(11).Compound 4 was isolated from natural resources for the first time,while the compounds 2-4,8-11 were obtained from the genus Eurya for the first time.The compound 10 showed moderate inhibitory activity on the proliferation of 5-8F cells by MTT method.