木犀草素-Zn(Ⅱ)配位温敏分子印迹聚合物的制备及其性能

Preparation and Properties of Luteolin-Zn(Ⅱ)Complex Thermo-sensitive Molecularly Imprinted Polymers

以木犀草素-Zn(Ⅱ)配合物为模板分子,丙烯酰胺为功能单体,N-异丙基丙烯酰胺为温敏单体,N,N′-亚甲基双丙烯酰胺为交联剂制备木犀草素-Zn(Ⅱ)配位温敏分子印迹聚合物(thermo-sensitive molecularly imprinted polymers,TMIPs),分离箬叶黄酮碳苷。结果表明,TMIPs最佳制备工艺为模板分子、功能单体、温敏单体、交联剂摩尔比1∶4∶16∶30,其低临界溶解温度约为32℃,吸附性能符合Langmuir吸附模型和准一级动力学模型,TMIPs对木犀草素吸附量为36.06μmol/g,印迹因子为3.18,相对于芹菜素和芦丁,对木犀草素选择系数分别达到1.83和2.18,对温度响应灵敏,易于控制解吸。TMIPs用于分离箬叶黄酮碳苷,对牡荆苷、异牡荆苷、荭草苷、异荭草苷吸附量分别为32.99、27.73、35.16、36.05μmol/g。

Thermo-sensitive luteolin-Zn(Ⅱ)complex molecularly imprinted polymers(TMIPs)were synthesized for the separation of C-glycosylflavones from Indocalamus tessellates leaves with luteolin-Zn(Ⅱ)complex as the template molecule,acrylamide as functional monomer,N-isopropylacrylamide as thermo-sensitive monomer,and N,N′-methylenebisacrylamide as cross-linker.The results showed that the optimal preparation condition of TMIPs was template molecule:functional monomer:thermo-sensitive monomer:cross-linker=1∶4∶16∶30(mole ratio).The lower critical solution temperature of TMIPs was about 32℃.The adsorption performance was consistent with the Langmuir adsorption model and the pseudo-first-order kinetic model.The adsorption capacity of TMIPs for luteolin reached 36.06μmol/g,and its imprinting factor was 3.18.Compared with apigenin and rutin,the selectivity coefficients for luteolin reached 1.83 and 2.18,respectively.TMIPs were sensitive to temperature and easy to control adsorption and desorption.TMIPs were applied for the separation of C-glycosylflavones from I.tessellates leaves,with adsorption capacity for vitexin,isovitexin,orientin,and isoorientin reaching 32.99,27.73,35.16,and 36.05μmol/g,respectively.