蓝莓GolS基因家族鉴定及非生物胁迫下的表达模式分析

Genome-wide identification and abiotic stress expression analysis of GolS gene family in Vaccinium corymbosum

肌醇半乳糖苷合成酶(Galactinol synthase,GolS)是催化棉子糖合成的关键酶,在植物抵御非生物胁迫中发挥着重要作用。为研究蓝莓Gol S的潜在功能,采用生物信息学方法鉴定蓝莓Gol S基因家族成员,使用q RT-PCR技术分析Vc Gol S在不同组织和非生物胁迫下的表达模式。结果显示,22个蓝莓Gol S基因相对分子量介于36.36~74.72 kDa,均具有GT8保守结构域,含有10种motif,分为5个亚家族。共线性和进化选择分析表明,蓝莓Gol S基因家族成员比较保守,在进化过程中受纯化选择。Vc Gol S基因启动子中含有大量植物生长发育、激素响应和非生物胁迫响应顺式作用元件。表达模式分析结果表明:6个Vc Gol S基因在不同组织中均有表达,表达量差异较大,在根和叶中表达量较高;在盐、干旱和ABA诱导胁迫下,Vc Gol S表达丰度各异,但以上调表达为主。本研究将为进一步开展Vc Gol S基因的功能研究和逆境响应机制提供参考。

Galactinol synthase is a key enzyme for RFOs synthesis and plays an important role in plant resistance to abiotic stress.To study the potential function of VcGolS,bioinformatics methods were used to identify VcGolS gene family,and qRT-PCR was used to analyze the expression patterns of the differentially expressed VcGolS genes in different tissue and abiotic stress.The results showed that the relative molecular weight of 22 VcGolS gene was between 36.36-74.72 kDa,all of them had GT8 conserved domain and contained 10 motifs,and were divided into five subfamilies.Collinearity and evolutionary selection analysis showed that VcGolS genes were conserved,and purify selected in the process of evolution.The promoter of VcGolS genes contained a large number of plant growth and development,hormone response and abiotic stress response cis-acting elements.Expression pattern analysis showed that the 6 VcGolS genes were expressed in different tissue,the expression amounts were higher in roots and leaves;the expression abundance of VcGolS varied under salt,drought,and ABA stress,and the up-regulated expression was predominant.The present study provides a reference for the further study of the biological functions and stress response mechanism of VcGolS genes under abiotic stress.