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外周血线粒体DNA、生长分化因子-15联合脑钠肽检测对心力衰竭的诊断价值

Diagnostic Value of Peripheral Blood Mitochondrial DNA,Growth Differentiation Factor-15 Combined with Brain Natriuretic Peptide in Patients with Heart Failure
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摘要 目的探讨外周血线粒体DNA(mtDNA)、生长分化因子-15(GDF-15)联合脑钠肽(BNP)检测对心力衰竭(HF)患者的诊断价值。方法连续纳入2019年8月-2020年1月于承德医学院附属医院心脏内科住院的心力衰竭患者74例作为试验组。其中按照纽约心功能分级(NYHA),NYHAⅡ级患者10例,NYHAⅢ级患者14例,NYHAⅣ级患者50例,同期住院心功能正常患者75例作为对照组。收集入选对象外周血标本,应用实时定量RT-PCR检测入选对象外周血mtDNA含量,应用ELISA法测量入选对象外周血GDF-15的含量,收集电化学发光法检测的BNP含量及一般临床资料,建立基线数据库。结果多因素回归分析显示,外周血GDF-15升高、mtDNA水平下降是心力衰竭的独立危险因素。与对照组比较,心力衰竭组外周血mtDNA的含量较低,GDF-15、BNP的水平较高,差异有统计学意义(P<0.05);ROC曲线分析显示,mtDNA、GDF-15、BNP、的曲线下面积分别是0.784、0.803、0.964,联合三者的曲线下面积是0.979(P<0.05)。结论心力衰竭患者外周血中mtDNA表达降低,GDF-15、BNP水平升高,BNP联合mtDNA、GDF-15可提高对心力衰竭的诊断能力。 Objective To explore the diagnostic value of peripheral blood mitochondrial DNA(mtDNA),growth differentiation factor-15(GDF-15)combined with brain natriuretic peptide(BNP)in patients with heart failure(HF).Methods A total of 74 patients with heart failure who were hospitalized in the Department of Cardiology,Affiliated Hospital of Chengde Medical University from August 2019 to January 2020 were continuously included as the experimental group.According to the New York Heart Association(NYHA),there were 10 patients with NYHAⅡ,14 patients with NYHAⅢ,50 patients with NYHAⅣ,and 75 patients with normal heart function as control group.Peripheral blood samples were collected from the selected subjects.Real-time quantitative RT-PCR was used to detect the content of mtDNA in peripheral blood of the selected subjects.The content of GDF-15 in peripheral blood of the selected subjects was measured by ELISA.The content of BNP detected by electrochemiluminescence and general clinical data were collected to establish a baseline database.Results Multivariate regression analysis showed that elevated peripheral blood GDF-15 and decreased mtDNA levels were independent risk factors for heart failure.Compared with the control group,the content of mtDNA in peripheral blood of the heart failure group was lower,and the levels of GDF-15 and BNP were higher(P<0.05).ROC curve analysis showed that the area under the curve of mtDNA,GDF-15,BNP was 0.784,0.803,0.964,respectively,and the area under the curve of the combination of the three was 0.979(P<0.05).Conclusion The expression of mtDNA in peripheral blood of patients with heart failure is decreased,and the levels of GDF-15 and BNP are increased.BNP combined with mtDNA and GDF-15 can improve the diagnostic ability of heart failure.
作者 黎百志 李晓慧 张爱文 LI Bai-zhi;LI Xiao-hui;ZHANG Ai-wen(Department of Cardiology,the Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei,China)
出处 《医学信息》 2023年第12期86-90,124,共6页 Journal of Medical Information
基金 河北省教育厅研究生创新资助项目(编号:CXZZSS2020130)。
关键词 线粒体DNA 生长分化因子-15 脑钠肽 心力衰竭 Mitochondrial DNA Growth differentiation factor-15 Brain natriuretic peptide Heart failure
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