刺玫果黄酮对胰岛素抵抗HepG2细胞糖代谢功能研究

Flavonoids from Rosa davurica Pall.on Glucose Metabolism Function in Insulin Resistant HepG2 Cells

为研究刺玫果黄酮处理对胰岛素抵抗人肝癌细胞HepG2(IR-HepG2)细胞糖代谢功能,以及关键酶活性的影响,以高浓度胰岛素诱导HepG2细胞建立IR-HepG2细胞模型;刺玫果黄酮处理细胞24 h,四甲基偶氮唑蓝(MTT)法检测细胞活性,并评价刺玫果黄酮处理对葡萄糖消耗量的影响;测定细胞内糖原积累水平和IR-HepG2细胞关键酶己糖激酶(HK)、丙酮酸激酶(PK)、葡萄糖-6-磷酸酶(G6Pase)、磷酸烯醇式丙酮酸羧激酶(PEPCK)活性。研究结果表明:以0.1μmol/L胰岛素处理HepG2细胞24 h成功建立IR-HepG2细胞模型;0.10~1.00 g/L刺玫果黄酮处理组细胞葡萄糖消耗率均大于18%,以培养24 h的细胞活性最好。与模型组相比,1.00 g/L刺玫果黄酮处理组使细胞糖原质量分数提高81.45%(P<0.05),HK、PK活性分别提高29.26%(P<0.05)、31.93%(P<0.05),PEPCK、G6PC活性分别降低30.82%(P<0.05)、7.32%(P<0.05)。刺玫果黄酮是可以通过调节IR-HepG2细胞关键酶来调节碳水化合物代谢的潜在生物活性化合物,该发现值得开展深入研究。

To study the effects of flavonoids from Rosa davurica Pall.on glucose metabolism and key enzyme activities in insulin-resistant HepG(IR-HepG2)cells,the HepG2 cells were induced by high concentration of insulin to established IR-HepG2 cell model.The cells were treated with R.davurica flavonoid for 24 h.The cell activity was detected by MTT method,and the effect of the glucose consumption treated by R.davurica flavonoid was evaluated.The intracellular glycogen accumulation levels and IR-HEPG2 cell key enzymes hexokinase(HK),pyruvate kinase(PK),glucose-6-phosphatase(G6Pase),phosphoenol acetate carboxylase(PEPCK)activities were determined.The results showed that IR-HepG2 cell model was successfully established by treating HepG2 cells with 0.1μmol/L insulin for 24 h.The glucose consumption rate of cells in the 0.10-1.00 g/L R.davurica flavonoid treatment group was all more than 18%,and the cell activity was best after cultured for 24 h.Compared with the model group,the 1.00 g/L R.davurica flavonoid treatment group increased the glycogen content by 81.45%(P<0.05),HK and PK viability increased by 29.26%(P<0.05)and 31.93%(P<0.05),and the activities of PEPCK and G6Pase decreased by 30.82%(P<0.05)and 7.32%(P<0.05),respectively.R.davurica flavonoids were potential bioactive compounds that could regulate carbohydrate metabolism by modulating key enzymes in IR-HepG2 cells warrants in-depth study.