党参多糖的大孔树脂纯化及其美白、吸湿保湿性能

Purification of Polysaccharides from Codonopsis pilosula by Macroporous Adsorption Resin and Its Whitening,Moisture Absorption and Moisturizing Properties

基于静态实验,比较了14种不同型号大孔吸附树脂S-8、ADS-F8、LSA-21、LSA-10、HP-20、ADS-17、NKA-9、DM130、AB-8、X-5、H103、D101、XAD-8和DA201对党参多糖的纯化效果,优选出较佳树脂ADS-F8进行动态吸附。在单因素试验基础上,以多糖保留率、脱色率、蛋白质脱除率的加权综合评分作为响应值,采用响应面法优化大孔树脂纯化党参多糖的工艺条件,并对纯化后的党参多糖进行美白活性、吸湿和保湿性能测试。研究结果表明:较佳纯化工艺条件为上样质量浓度1 g/L、洗脱液体积150 mL、流速100 mL/h、上样量150 mL,此条件下纯化的综合评分为(73.12±0.06)%(n=3),与预测值73.02%接近。纯化多糖具有良好的美白活性,对酪氨酸酶单酚酶的半数抑制质量浓度(IC_(50))值为3.20 g/L,与美白剂苯乙基间苯二酚的抑制作用相当;对二酚酶的IC_(50)值为7.96 g/L,高于苯乙基间苯二酚的0.73 g/L;当空气湿度为43%时,纯化多糖表现出较强的吸湿性,12 h的吸湿率最高,达123.01%;纯化多糖、粗多糖及其与甘油体积比1∶1混合物在4种空气湿度下均具有较高的保湿性,特别是混合物的保湿性更佳,12 h内的保湿率达到99.95%,保湿性能明显优于纯化多糖和粗多糖。

On the basis of static experiments,the purification effects of polysaccharides on 14 kinds of macroporous adsorption resins(S-8,ADS-F8,LSA-21,LSA-10,HP-20,ADS-17,NKA-9,DM130,AB-8,X-5,H103,D101,XAD-8,DA201)from Codonopsis pilosula(Franch.)Nannf.extraction were compared,and the better resin ADS-F8 was selected for dynamic adsorption.Based on the single factor experiments,the weighted comprehensive score of polysaccharides retention rate,decolorization rate and protein removal rate were used as the response values,and the response surface methodology was used to optimize the purification conditions of C.pilosula polysaccharide by macroporous resin.Meanwhile,the whitening activity,moisture absorption and moisture retention of the purified polysaccharides were tested.The results showed that the optimal purification conditions were as follows:loading concentration of 1 g/L,eluting volume of 150 mL,flow rate of 100 mL/h,and sample volume of 150 mL.Under these conditions,the comprehensive score of purification was(73.12±0.06)%(n=3),which was close to the predicted value of 73.02%.The purified polysaccharides had favorable whitening activity,and the half inhibitory mass concentration(IC_(50))value of tyrosinase monophenase was 3.20 g/L,which was similar to the inhibitory effect of whitening agent phenylethylresorcinol.The IC_(50) value of bisphenolase was 7.96 g/L,which is higher than phenylethylresorcinol of 0.73 g/L.When the air humidity was 43%,the purified polysaccharide showed strong hygroscopicity,and the highest moisture absorption rate was 123.01%at 12 h.In four air humidity conditions,the purified polysaccharides,crude polysaccharides and their mixture with a volume ratio 1∶1 to glycerol all had high moisture retention,especially,the moisturizing property of the mixture was better,and the moisturizing rate reached 99.95%within 12 h,which was significantly better than that of purified polysaccharide and crude polysaccharide.