cobas e 411电化学发光全自动免疫分析仪和贝克曼UniCel DxI 800化学发光仪检测血清β-HCG及孕酮的比对分析

Detection of Serum Using cobas e 411 Electrochemiluminescence Fully Automated Immunoassay Analyzer and Beckmann UniCel DxI 800 Chemiluminescence Analyzerβ-Comparative Analysis of HCG and Progesterone

目的:对比cobas e 411电化学发光全自动免疫分析仪和贝克曼UniCel DxI 800化学发光仪在血清β-HCG以及孕酮检测中的应用价值。方法:纳入2021年1月~2021年6月于本院就诊的女性患者200例作为研究对象,经超声确诊将200例患者分成未孕组(n=105)、早孕组(n=37)、异位妊娠组(n=58),对所有患者血液样本均进行贝克曼UniCel DxI 800化学发光仪和cobas e 411电化学发光全自动免疫分析仪检测,比较两种不同系统检测的血清β-HCG以及孕酮水平。结果:cobas e 411电化学发光全自动免疫分析仪检测下,未孕组患者、早孕组患者、异位妊娠组患者的血清β-HCG分别为:(2.91±0.40)mIU/mL、(31275.36±6138.43)mIU/mL、(180.39±80.65)mIU/mL,孕酮分别为:(1.72±0.78)nmol/L、(113.26±46.82)nmol/L、(13.05±7.04)nmol/L;贝克曼UniCel DxI 800化学发光仪检测下,未孕组患者、早孕组患者、异位妊娠组患者的血清β-HCG分别为:(2.90±0.42)mIU/mL、(30986.16±6149.79)mIU/mL、(180.47±79.82)mIU/mL,孕酮分别为:(1.73±0.79)nmol/L、(114.16±47.46)nmol/L、(13.13±7.10)nmol/L。未孕组、早孕组、异位妊娠组患者在通过两种检测系统检测下得到的血清β-HCG、孕酮结果组间比较差异均无统计学意义(P>0.05)。结论:通过cobas e 411电化学发光全自动免疫分析仪和贝克曼UniCel DxI 800化学发光仪对患者的血清β-HCG、孕酮结果进行检测,发现两种检测系统的准确性高,相关性良好,可以有效避免结果差异性给医生带来诊断困扰,得到的数据和临床要求均相符。

Objective:To compare the performance of cobas e 411 electrochemiluminescence fully automated immunoassay analyzer and Beckmann UniCel DxI 800 chemiluminescence analyzer in serumβ-the application value of HCG and progesterone detection.Methods:200 female patients who visited our hospital from January 2021 to June 2021 were included as the study subjects.After ultrasound diagnosis,200 patients were divided into an unpregnant group(n=105),an early pregnancy group(n=37),and an ectopic pregnancy group(n=58).Blood samples from all patients were tested using Beckman UniCell DxI 800 chemiluminescence instrument and cobas e 411 electrochemical luminescence fully automated immunoassay instrument,and the serum detected by two different systems was comparedβ-HCG and progesterone levels.Results:The serum of patients in the non pregnancy group,early pregnancy group,and ectopic pregnancy group was detected by cobas e 411 electrochemical luminescence fully automatic immunoassay analyzerβ-HCG was(2.91±0.40)mIU/mL,(31275.36±6138.43)mIU/mL,(180.39±80.65)mIU/mL,and progesterone was(1.72±0.78)nmol/L,(113.26±46.82)nmol/L,and(13.05±7.04)nmol/L,respectively;Serum levels of patients in the non pregnancy group,early pregnancy group,and ectopic pregnancy group were detected using the Beckman UniCel DxI 800 chemiluminescence instrumentβ-HCG was(2.90±0.42)mIU/mL,(30986.16±6149.79)mIU/mL,(180.47±79.82)mIU/mL,and progesterone was(1.73±0.79)nmol/L,(114.16±47.46)nmol/L,and(13.13±7.10)nmol/L.Serum obtained from patients in the non pregnancy group,early pregnancy group,and ectopic pregnancy group through two detection systems There was no statistically significant difference inβ-HCG and progesterone results between groups(P>0.05).Conclusion:By using cobas e 411 electrochemiluminescence fully automatic immunoassay analyzer and Beckmann UniCel DxI 800 chemiluminescence analyzer,the serum of patients was measuredβ-HCG and progesterone results were tested and it was found that the two detection systems have high accuracy and good correlation,which can effectively avoid diagnostic difficulties caused by differences in results for doctors.The obtained data and clinical requirements are consistent.