岩白菜素对脂多糖诱导小胶质细胞炎症反应的抑制作用及其分子机制

Inhibitory effect of bergenin on LPS-induced inflammatory response of microglia and its molecular mechanism

目的探讨岩白菜素对脂多糖(LPS)诱导的BV2小胶质细胞炎症反应模型的影响以及其机制是否与调控小胶质细胞M1/M2表型极化相关。方法BV2小胶质细胞分为对照组、LPS组、LPS+低剂量岩白菜素组、LPS+高剂量岩白菜素组,LPS+低剂量岩白菜素组、LPS+高剂量岩白菜素组分别加入10μmol/L、100μmol/L的岩白菜素预处理2 h后与LPS组一起加入LPS刺激诱导炎症反应模型,对照组正常培养不做任何处理。各组置于倒置显微镜下观察细胞形态变化,Western blotting法检测细胞M1型小胶质细胞分泌型标志物一氧化氮诱导合酶(iNOS)、M2型小胶质细胞分泌型标志物精氨酸1(Arg-1)蛋白表达。结果对照组细胞呈梭形,胞体小,有较少的分支形成;LPS组细胞胞体变大,细胞分支增多,呈阿米巴样;LPS+低剂量岩白菜素组、LPS+高剂量岩白菜素组细胞形态均较LPS组有改善,胞体变圆,分支减少。细胞iNOS蛋白表达对照组、LPS+高剂量岩白菜素组、LPS+低剂量岩白菜素组>LPS组;细胞Arg-1蛋白表达LPS组>iNOS蛋白表达对照组、LPS+高剂量岩白菜素组、LPS+低剂量岩白菜素组(P均<0.05)。结论岩白菜素可抑制LPS诱导的小胶质细胞炎症反应,该作用可能通过促进小胶质细胞向M2表型极化实现。

Objective To investigate the effect of bergenin(BGN)on the lipopolysaccharide(LPS)-induced inflammatory response model of BV2 microglia and whether its mechanism is related to the regulation of M1/M2 phenotype polarization of microglia.Methods BV2 microglial cells were divided into the control group,LPS group,LPS+low-dose BGN group,and LPS+high-dose BGN group.Cells in the LPS+low-dose and LPS+high-dose BGN groups were pretreated with 10μmol/L and 100μmol/L bergenin for 2 h,respectively,and cells in the LPS group,LPS+low-dose BGN group and LPS+high-dose BGN group were added with LPS to stimulate and induce inflammatory response models.Cells in the control group were cultured normally without any treatment.The morphological changes of cells in each group were observed under an inverted microscope.The expression of inducible nitric oxide synthase(iNOS)in M1 microglia and arginine 1(Arg-1)in M2 microglia were detected by Western blotting.Results In the control group,the cells were fusiform,with small cell bodies and fewer branches.The cell body of the LPS group was enlarged and the cell branches increased.Compared with the LPS group,the cell morphology of the LPS+low-dose BGN group and LPS+high-dose BGN group was improved,and the cell bodies became round and the branches were reduced.The iNOS protein expression was higher in the control group,LPS+high-dose BGN group,and LPS+low-dose BGN group than in the LPS group;and the Arg-1 protein expression was higher in LPS group than in the control group,LPS+high-dose BGN group and LPS+low-dose BGN group(all P<0.05).Conclusion Bergenin can inhibit the LPS-induced inflammatory response of microglia,possibly by promoting the polarization of microglia towards the M2 phenotype.