基于网络药理学的补肾活血汤治疗糖尿病勃起功能障碍的作用机制研究

Exploring the mechanism of Bushen Huoxue soup for the treatment of diabetes mellitus erectiledysfunction based on the mechanism of network pharmacology

目的使用网络药理学的方法,探寻补肾活血汤治疗糖尿病勃起功能障碍的分子机制。方法采用TCMSP数据库及Swiss数据平台筛选出补肾活血汤方中的主要有效化合物及其靶点;运用Cytoscape 3.8绘制补肾活血汤成分-靶点网络;通过Genecards、OMIM及Drugbank数据库检索糖尿病勃起功能障碍的相关靶点;通过韦恩图得到补肾活血汤方、糖尿病勃起功能障碍的共同靶点;使用String平台构建化合物-疾病共同靶点的蛋白互相作用(PPI)网络;使用Metascape数据库进行GO富集分析和KEGG通路分析。结果本研究共获得补肾活血汤42个活性成分,427个相关靶点,糖尿病、勃起功能障碍相关靶点分别为736、743个,补肾活血汤调节糖尿病勃起功能障碍的作用靶点50个,再次筛选得到核心共同靶点10个;GO富集分析结果显示,补肾活血汤调节糖尿病勃起功能障碍的生物学过程主要包括生殖器、前列腺和性腺发育等。KEGG通路分析得到核心信号通路3条。结论补肾活血汤可能作用于IGFBP3、TP53、ESR1等靶点,途经p53通路治疗糖尿病勃起功能障碍。

Objective To explore the molecular mechanism of Bushenhuoxue soup for the treatment of diabeties mellitus erectile dysfunction using a network pharmacology approach.Methods The traditional Chinese medicine systems pharmacology(TCMSP)database and Swiss data platform were used to screen the main efective compounds and their targets in Bushen Huoxue soup;Cytoscape 3.8 was used to map the drug component-target network;Genecards,online mendelian inheritance in man(OMIM)and Drugbank databases were used to retrieve the targets related to diabetes melitus erectile dysfunction;Venn diagrams were used to obtain the common targets of Bushen Huoxue soup formula and diabetes mellitus erectile dysfunction.The String platformn was used to construct protein-protein interaction(PPI)networks of compound disease common targets.The Metascape database was used for gene ontology(CO)analysis and the kyoto encyelopedia of genes and genomes(KECC)pathway analysis.Results In this study,42 active ingredients and 427 related targets were obtained,736 targets related to diabetes and 743 tagets related to erectile dysfunction,and 50 targets related to.Bushen Huoxue soup,and 10 core common targets were obtained by re-screening.GO enrichment analysis showed that the biological process of regulating diabetes mellitus erectile dysfunction by Bushen Huoxue soup mainly includes genital,prostate and gonad development.3 core signal pathways were obtained by KEGG pathway analysis.Conclusions Bushen Huoxue soup may act on ICFBP3,TP53,ESRI and other targets,and treat diabetes mellitus erectile dysfunction through p53 pathway.