滇龙胆花化学成分的分离与鉴定

Isolation and Identification of Chemical Constituents of The Flowers of Cultivated Gentiana rigescens Franch.

目的:对滇龙胆花的化学成分进行了初步研究,明确滇龙胆花的化学成分并加以充分利用,为最大限度地提高滇龙胆种植产业效益提供科学依据。方法:采用硅胶、葡聚糖凝胶LH-20等色谱法分离化合物,并通过与标准品TLC对照、核磁共振、质谱数据鉴定化合物的结构。结果:从滇龙胆花体积分数为95%的乙醇溶液提取物中分离鉴定了12个化合物,主要为小极性的三萜脂肪酸酯酸,含量最大的是乌苏烷型三萜。分别鉴定为软脂酸(1)、古柯二醇-3-棕榈酸酯(2)、羽扇豆醇棕榈酸酯(3)、α-香树脂醇(4)、熊果醇-3-棕榈酸酯(5)、β-谷甾醇(6)、熊果酸(7)、胡萝卜苷(8)、2α-羟基-熊果酸(9)、β-香树脂醇棕榈酸酯(10)、α-香树脂醇棕榈酸酯(11)、β-香树脂醇(12)。结论:对滇龙胆花化学成分进行研究,分离鉴定了12个单体化合物,化合物1~6、8~12均为首次从龙胆花中分离得到。

Objective This research stuied the chemical constituents of the flowers of cultivated Gentiana rigescens Franch.,in order to identify the chemical compositions of Gentiana rigescens Franch.and use it,to improve the value.Methods the compounds were isolated by chromatography such as Silica gel,Dextran gel LH-20,and identified the structure by the physicochem-ical property and spectral data.Results 12 compounds were isolated from the alcoholic extracts of the flowers of cultivated Gentiana rigescens Franch..They were identified as:palmitic acid(1),Erythrodiol-3-palmitate(2),Lupeol palmitate(3),α-Amyrin(4),Uvaol-3-palmitate(5),β-Sitosterol(6),Ursolic acid(7),Daucosterol(8d),2α-hydroxyl-ursolic acid(9),β-Amyrin palmitate(10),α-Amyrin palmitate(11)andβ-Amyrin(12).Conclusion The compounds of1-6、8-12were isolated from the flowers of Gentiana for the first time.