丹酚酸B促进卵巢早衰小鼠卵泡发育的研究

Salvianolic acid B promotes follicular development in mice with premature ovarian failure

目的:探究丹酚酸B对卵巢早衰模型小鼠卵泡发育的影响。方法:选取27只6周龄雌性昆明小鼠,按照随机数字表法分为对照组、模型组和实验组,每组9只,对每只小鼠均给药,定期测量体重,给药结束后收集小鼠血清和卵巢组织。ELISA检测血清E2、AMH和FSH水平;HE染色观察卵巢窦状卵泡;免疫组化检测颗粒细胞PCNA的表达;TUNEL检测卵巢颗粒细胞凋亡情况;实时荧光定量PCR和Western blot测定卵巢组织中Bcl-2和Bax mRNA表达和其蛋白表达情况。结果:模型组小鼠体重明显低于对照组和实验组(P<0.05);与模型组比较,实验组血清E2和AMH水平升高且FSH水平降低,窦状卵泡数量增多,颗粒细胞中PCNA与Bcl-2表达增加,同时Bax表达减少,凋亡细胞数量减少(P<0.05)。结论:丹酚酸B能抑制卵巢早衰小鼠颗粒细胞凋亡,促进卵泡发育。

Objective:To investigate the effect of salvianolic acid B on follicular development in premature ovarian failure mice.Methods:27 six-week-old female Kunming mice were divided into the control group by the table of random number,model group and experimental group,each with nine mice.Each mouse was given the drug,weight was measured at regular intervals,and serum and ovarian tissue were collected after administration.Serum E2,AMH and FSH levels were detected by ELISA;HE staining was used to observe ovarian sinusoidal follicles;the expression of PCNA in granulosa cells was detected by immunohistochemistry;ovarian granulosa cell apoptosis was detected by TUNEL;the mRNA expressions of Bcl-2 and Bax,and their protein expressions were determined by PCR and Western blot.Results:The body weight of mice in the model group was significantly lower than that in control group and experimental group(P<0.05);compared with the model group,serum E2 and AMH were increased and FSH levels were decreased in experimental group,and the number of sinus follicles was increased,the expressions of PCNA and Bcl-2 in granulosa cells were increased,and the apoptosis of granulosa cells was decreased,the expression of Bax was decreased at the same time(P<0.05).Conclusion:Salvianolic acid B can in granulosa cell apoptosis and promote follicle development in premature ovarian failure mice.