摘要
Cell metabolism plays vital roles in organismal development,but it has been much less studied than transcriptional and epigenetic control of developmental programs.The difficulty might be largely attributed to the lack of in situ metabolite assays.Genetically encoded fluorescent sensors are powerful tools for noninvasive metabolic monitoring in living cells and in vivo by highly spatiotemporal visualization.Among all living organisms,the NAD(H)and NADP(H)pools are essential for maintaining redox homeostasis and for modulating cellular metabolism.Here,we introduce NAD(H)and NADP(H)biosensors,present example assays in developing organisms,and describe promising prospects for how sensors contribute to developmental biology research.
基金
This research is supported by National Key Research and Development Program of China(2019YFA0904800 to Y.Zhao)
NSFC(32030065,92049304,32121005 to Y.Zhao,31901033 to T.L.)
Research Unit of New Techniques for Live-cell Metabolic Imaging(Chinese Academy of Medical Sciences,2019RU01,2019-I2M-5-013 to Y.Zhao)
Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism(Y.Zhao)
Innovative research team of high-level local universities in Shanghai,the Shanghai Science and Technology Commission(19YF1411300 to T.L.)
China Postdoctoral Science Foundation(2019 M651413 to T.L.)
the State Key Laboratory of Bioreactor Engineering,the Fundamental Research Funds for the Central Universities.
