黄柏碱对大鼠超负荷运动后抗氧化能力和炎症的影响

Effects of Phellodendrine on Antioxidation and Inflammation in Rats After Overload Exercise

本研究考察了黄柏碱(phellodendrine,PHE)对超负荷运动(OLE)诱导的大鼠心肌损伤和氧化应激的影响。将大鼠分成5组(n=20),以递增负荷游泳建立的OLE大鼠模型。检测了各组大鼠的心功能参数(LVPWd,LVPWs,LVIDs,EF和FS)、血清心肌损伤指标(LDH,CK和cTnI)和心肌组织氧化应激指标(SOD,CAT和MDA)。通过HE染色观察心肌组织形态。通过RT-qPCR检测心肌组织中TNF-α和IL-6 mRNA水平。通过Western blot检测心肌组织中Nrf2(细胞核)、Keap1、HO-1、p-NF-κB p65和NF-κB p65的蛋白水平。研究结果表明:与OLE组比较,OLE+L-PHE组、OLE+M-PHE组和OLE+H-PHE组的LVPWd、LVPWs和LVIDs降低,EF和FS升高,LDH、CK和cTnI水平降低,心肌损伤减轻(P<0.05)。与OLE组比较,OLE+L-PHE组、OLE+M-PHE组和OLE+H-PHE组的SOD和CAT升高,MDA降低,TNF-α和IL-6 mRNA水平降低(P<0.05)。与OLE组比较,OLE+L-PHE组、OLE+M-PHE组和OLE+H-PHE组的Nrf2(细胞核)和HO-1蛋白相对表达量升高,Keap1蛋白相对表达量降低,NF-κB p65磷酸化水平降低(P<0.05)。综上所述,黄柏碱可能通过激活Nrf2信号通路同时抑制NF-κB信号通路改善超负荷运动大鼠的心肌组织抗氧化和抗炎能力,从而减轻超负荷运动诱导的心肌损伤。

To investigate the effects of phellodendrine(PHE)on myocardial injury and oxidative stress induced by overload exercise(OLE)in rats.The rats were divided into 5 groups and OLE rats established by increasing load swimming.Cardiac function parameters(LVPWd,LVPWs,LVIDs,EF and FS),serum myocardial injury indexes(LDH,CK and cTnl)and myocardial tissue oxidative stress indexes(SOD,CAT and MDA)were measured.The morphology of myocardium was observed by HE staining.The levels of TNF-αand IL-6 mRNA in myocardial tissue were detected by RT-qPCR.The protein levels ofNrf2(nucleus),Keap1,HO-1,p-NF-kB p65 and NF-kB p65 in myocardial tissue were detected by Western blot.Compared with OLE group,LVPWd,LVPWs and LVIDs decreased,EF and FS increased,LDH,CK and cTnI levels decreased and myocardial injury alleviated in OLE+L-PHE,OLE+M-PHE and OLE+H-PHE groups(P<0.05).Compared with OLE group,SOD and CAT in OLE+L-PHE group,OLE+M-PHE group and OLE+H-PHE group increased,MDA decreased,TNF-αand IL-6 mRNA levels decreased(P<0.05).Compared with OLE group,the relative expression of Nrf2(nucleus)and HO-1 protein in OLE+L-PHE group,OLE+M-PHE group and OLE+H-PHE group increased,while the relative expression of Keapl pro tein decreased,and the phosphorylation level of NF-kB p65 decreased(P<0.05).To sum up,Phellodendrine may improve the ability of anti-oxidation and anti-inflammation of myocardial tissue of overload exercise rats by activating Nrf2 signal pathway and inhibiting NF-kB signal pathway,so as to reduce myocardial injury induced by overload exercise.