代谢综合征痰证的危险因素:EXT2基因rs11037909/rs1113132/rs3740878-T等位基因、BMI与TG

Risk Factors of Phlegm Syndrome in Metabolic Syndrome:EXT2 Gene rs11037909/rs1113132/rs3740878-T Allele,BMI and TG

目的 探讨EXT2基因的单核苷酸多态性(SNPs)对代谢综合征(MS)痰证易感性的影响。方法 选择2013年11月—2020年12月福建中医药大学附属第三人民医院治未病中心、体检中心,福建中医药大学附属晋江市中医院内分泌科就诊的MS患者849例,运用证素辨证方法将MS患者分成MS痰证组(641例)和非痰证组(208例),采用SNPscan多重SNP分型技术检测EXT2的rs11037909、rs1113132、rs3740878基因型,对EXT2基因的SNPs、体重指数(BMI)和甘油三酯(TG)进行Logistic回归分析。结果 (1)MS痰证组的BMI、TG与痰证素呈正相关(P<0.05)。(2)携带EXT2基因rs11037909、rs1113132、rs3740878基因型(CT/TC或CT+CC)、(CC或CG+CC)、(CC或CT+CC)者患痰证的风险是携带TT、GG、TT基因型者的0.562或0.666倍、0.559或0.645倍、0.561或0.650倍。(3)携带EXT2 rs11037909 CT/TC+CC、rs1113132 CG/GC+CC、rs3740878 CT/TC+CC基因型的超重肥胖者患痰证的风险是携带TT、GG、TT基因型的超重肥胖者的0.642、0.618、0.624倍。(4)携带EXT2 rs11037909 CT/TC+CC基因型的高TG者患痰证的风险是携带TT基因型的高TG者的0.544倍;携带EXT2 rs1113132 CG/GC+CC、rs3740878 CT/TC+CC基因型的无高TG者患痰证的风险是携带GG、TT基因型的高TG者的0.524、0.531倍。(5)携带EXT2 rs11037909/rs1113132/rs3740878 CT/TC+CC或CG/GC+CC基因型的无超重肥胖、无高TG者患痰证(轻)的风险是携带TT或GG基因型的超重肥胖、高TG者的0.102倍。结论 以T/G等位基因为参照,EXT2基因rs11037909/rs1113132/rs3740878-C等位基因与非痰证相比是MS痰证的保护因素,EXT2基因rs11037909/rs1113132/rs3740878-T等位基因、BMI与TG均是MS痰证的危险因素,会增加痰证的易感性,三者交互作用后患痰证的风险比无危险因素者高。

Objective To investigate the effect of single nucleotide polymorphisms(SNPs)of EXT2 gene on the susceptibility of metabolic syndrome(MS)with phlegm syndrome.Methods From November 2013 to December 2020,a total of 849 patients with MS who were treated in the Pre Treatment Center and Physical Examination Center of the Third People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,and the Endocrine Department of Jinjiang Hospital of Traditional Chinese Medicine Affiliated to Fujian University of Traditional Chinese Medicine were selected.MS patients were assigned to MS phlegm syndrome group(641 cases)and non-phlegm syndrome group(208 cases)by using syndrome differentiation method.SNPscan multiple SNP typing technique was used to detect rs11037909,rs1113132 and rs3740878 genotypes of EXT2.Logistic regression analysis was performed on gene SNPs,body mass index(BMI)and triglyceride(TG).Results(1)BMI,TG and phlegm syndrome elements were positively correlated in MS phlegm syndrome group(P<0.05).(2)The risk of developing phlegm syndrome in patients with EXT2 rs11037909 CT/TC or CT+CC,rs1113132 CC or CG+CC,rs3740878 CC or CT+CC genotypes was 0.562 or 0.666 times,0.559 or 0.645 times,0.561 or 0.650 times than those with TT,GG and TT genotypes.(3)The risk of developing phlegm syndrome in overweight or obese individuals with EXT2 rs11037909 CT/TC+CC,rs1113132 CG/GC+CC,rs3740878 CT/TC+CC genotypes was 0.642 times and 0.618 times and 0.624 times than those with TT,GG,and TT genotypes.(4)The risk of phlegm syndrome in patients with high TG carrying EXT2 rs11037909 CT/TC+CC genotype was 0.544 times than that in high TG patients carrying TT genotype;The risk of developing phlegm syndrome in the non-TG patients with EXT2 rs1113132 CG/GC+CC,rs3740878 CT/TC+CC genotype was 0.524 times and 0.531 times than those high TG patients with GG or TT genotype.(5)The risk of developing phlegm syndrome(mild)in patients with EXT2 rs11037909/rs1113132/rs3740878 CT/TC+CC or CG/GC+CC genotypes without overweight,obesity and high TG was 0.102 times than those with TT or GG genotypes who were overweight or obese and had high TG.Conclusion Taking T/G allele as reference,EXT2 gene rs11037909/rs1113132/rs3740878-C allele is a protective factor for phlegm syndrome compared with non-phlegm syndrome,EXT2 gene rs11037909/rs1113132/rs3740878-T allele,both BMI and TG are risk factors of phlegm syndrome,which will increase the susceptibility of phlegm syndrome.