单克隆抗体竞争ELISA和FAVN检测狂犬病病毒免疫抗体的对比

Comparative Study on Monoclonal Antibody Competition ELISA and FAVN for Detection of Rabies Virus Immune Antibodies

为对比狂犬病荧光抗体病毒中和试验(FAVN)和单克隆抗体竞争酶联免疫吸附试验(ELISA)检测方法的相关性,用2种血清抗体检测方法分别检测在深圳市收集的798份宠物犬血清样品,利用McNemar检验和Kappa一致性检验对比研究2种方法的检测结果。结果显示:以FAVN方法检测结果为标准,单克隆抗体竞争ELISA方法的符合率为96.7%(95%CI:95.3%~97.8%),敏感性为97.6%(95%CI:96.2%~98.5%),特异性为84.9%(95%CI:72.9%~92.7%),约登指数为0.825。经McNemar检验,2种血清抗体检测方法的检测结果差异不具有统计学意义(P=0.078>0.05);经Kappa一致性检验,2种血清抗体检测方法检测结果不一致是小概率事件(P<0.001),且两者的检测结果一致性高(Kappa系数=0.758)。结果表明,用单克隆抗体竞争ELISA方法代替FAVN用于犬狂犬病病毒免疫抗体的大规模监测具有一定的可行性。

Atotal of 798 pet dog serum samples collected in Shenzhen were detected by fluorescent antibody virus neutralization test(FAVN)and monoclonal antibody competitive enzyme-linked immunosorbent assay(ELISA)for comparison of the two methods by McNemar test and Kappa consistency test.The results showed that,taking the FAVN test results as the standard,ELISA test results were 96.7%(95%CI:95.3%-97.8%)coincident,with the sensitivity of 97.6%(95%CI:96.2%-98.5%),specificity of 84.9%(95%CI:72.9%-92.7%),and Jordan index of 0.825.McNemar Test results revealed that,no significant difference(P=0.078>0.05)was observed from the results of the two methods.Kappa consistency test revealed that the inconsistent results of the two methods were determined as a small probability(P<0.001),instead,a high consistency(Kappa coefficient=0.758)was observed.In conclusion,FAVN could be replaced by monoclonal antibody competition ELISA that might be used for intensive monitoring for canine rabies immune antibodies.