基因表达数据库对冠心病关键调控基因的生物信息学分析

Bioinformatics analysis of hub regulatory genes of coronary heart disease using Gene Expression Omnibus database

目的 应用基因表达(GEO)数据库对冠心病患者相关基因进行研究分析,探索新的冠心病潜在调控机制。方法 检索GEO数据库,选择冠心病患者和对照者的相关基因表达谱数据。导入处理后的冠心病患者的GSE71226数据集,利用GEO2R分析鉴定冠心病和健康人的差异表达基因(DEGs),通过分析确定冠心病患者和对照者的差异基因。采用R语言ggplot2包进行可视化相关分析。结果 共筛选出575个DEGs。进一步构建了一个蛋白质-蛋白质相互作用(PPI)网络,以可视化DEGs和识别中心基因。基因本体论通路富集分析显示,上调和下调DEGs的各排名前15的信号通路参与了胰岛素分泌、心脏传导系统、生长激素分泌等相关的生物过程;京都基因与基因组百科通路富集分析显示,上调和下调DEGs主要与序列特异性DNA复制、环状腺苷单磷酸酯(cAMP)、脂肪消化吸收酶等相关的信号通路等有关。进一步构建了PPI网络互作图,显示五个中心基因(SST、BBS10、CCK、POMC、HSPA9),其中SST与冠心病的预后密切相关。结论 冠心病的关键调控基因包括SST、BBS10、CCK、POMC、HSPA9,可能通过双链DNA结合、cAMP、脂肪消化吸收酶等相关的信号通路参与冠心病发病。

Objective To analyze genes related to coronary heart disease(CHD)patients and to explore potential new mechanisms for CHD using Gene Expression Omnibus(GEO)database.Methods The gene expression profile dataset of CHD patients and healthy controls was searched and retrieved from the GEO database.Differentially expressed genes(DEGs)between CHD patients and controls were identified using GEO2R analysis of the processed GSE71226 dataset.Visual correlation analysis was performed using the R language ggplot2 package.Results A total of 575 DEGs were screened.A protein-protein interaction(PPI)network was further constructed to visualize the DEGs and identify the genes.Gene ontology pathway enrichment analysis showed that the top 15 signaling pathways upregulating and downregulating DEGs were involved in biological processes,including insulin secretion,cardiac conduction system,growth hormone secretion,etc.Kyoto encyclopedia of genes and genomes pathway enrichment analysis showed that upregulating and downregulating DEGs were mainly related to sequence-specific DNA replication,cyclic adenosine monophosphate(cAMP),fat digestion and absorption,and other related signaling pathways.PPI network interaction diagram showed five central genes(SST,BBS 10,CCK,POMC,HSPA 9),among which SST was closely related to the pathogenesis of CHD.Conclusion The hub regulatory genes of CHD include SST,BBS 10,CCK,POMC,HSPA 9,probably participating in the pathogenesis of CHD through DNA replication,cAMP,fat digestion and absorption,and other related signal pathways.