口服五维赖氨酸葡萄糖中盐酸赖氨酸含量的研究

Research on Content of Lysine Hydrochloride in Oral Five Dimensional Lysine Glucose

建立氨基酸分析仪测定口服五维赖氨酸葡萄糖中盐酸赖氨酸含量的分析方法。样品经0.1 mol/L盐酸溶液溶解,采用氨基酸分析仪钠系统进行程序洗脱,LCAK06/Na色谱柱分离,茚三酮溶液衍生显色,在570 nm波长下测定赖氨酸色谱峰,采用外标法峰面积定量,保留时间定性。表明,盐酸赖氨酸在1.15~34.62μg/mL有良好的线性关系(r=0.9999),回收率为96.9%,相对标准偏差为1.5%(n=6)。采用氨基酸分析仪测定口服五维赖氨酸葡萄糖中盐酸赖氨酸的含量方法简单、便捷、准确性高。

Objective To establish a method to Lysine hydrochloride content of Oral Five Vitamins Lysine and Glucose by amino acid analyzer.Methods The sample was dissolved in 0.1 mol·L^(-1)hydrochloric acid solution,the program was eluted with an amino acid analyzer sodium system,separated by LCAK06/Na column,and colorimetrically derived from ninhydrin solution,and the Lysine chromatographic peaks was measured at a wavelength of 570 nm,quantification of peak area by external standard method,retention time qualitative.Results Taurine had a good linear relationship in the concentration range of 1.15~34.62 g/mL(r=1.0000)with a recovery rate of 96.9%and a relative standard deviation of 1.5%(n=9).Conclusion This method is simple,convenient,accurate and suitable for the quantitative analysis of taurine in infant milk powder.