玉米低聚肽制备条件优化

Optimization of Preparation Conditions for Maize Oligopeptide

以玉米湿蛋白为原料,通过酸性蛋白酶水解制备玉米低聚肽。以水解度作为指标进行单因素试验和正交试验,对影响蛋白水解的因素(底物浓度、pH、加酶量、温度、周期)进行条件优化。结果显示,最佳酶解条件为底物浓度6%、p H 3.6、加酶量10%、温度50℃、反应周期28 h。在此条件下,玉米湿蛋白水解度为72.30%。试验结果为玉米低聚肽的工业化生产提供技术支持。

The maize oligopeptide was prepared from maize wet protein by acid protease hydrolysis.The degree of hydrolysis was used as the index for single factor test and orthogonal experiment,and the factors affecting proteolysis(substrate concentration,pH,enzyme amount,temperature and period)were optimized.The results showed that the optimum conditions were substrate concentration 6%,pH 3.6,enzyme amount 10%,temperature 50℃,and reaction period 28 h.Under this condition,the degree of hydrolysate was 72.30%.The technical support for the industrial production of maize oligopeptide was provided.