白藜芦醇对铜绿假单胞菌群体感应抑制作用

Inhibition Effect of Resveratrol on Quorum Sensing in Pseudomonas aeruginosa

铜绿假单胞菌以群体感应(quorum sensing, QS)机制调控生物膜、胞外多糖、绿脓素等毒性因子表达,因此可通过群体感应抑制(quorum sensing inhibition, QSI)原理降低或抑制铜绿假单胞菌上述毒性因子的表达。为研究白藜芦醇作为群体感应抑制剂对铜绿假单胞菌PAO1中群体感应调控性状生物膜、胞外多糖、绿脓素产生的抑制作用,探究白藜芦醇对紫色杆菌CVO26产紫色素的抑制作用;对PAO1生物膜、胞外多糖、绿脓素等产生量进行测定,以及白藜芦醇与阿奇霉素对PAO1的协同抑菌作用进行研究。结果表明:白藜芦醇浓度40和60μg/mL时,其对CVO26生长无抑制作用,但对CVO26紫色素产生具有明显抑制作用;白藜芦醇浓度20~80μg/mL对PAO1生长无抑制作用,但对PAO1生物膜、胞外多糖、绿脓素产生均有抑制作用;40μg/mL白藜芦醇与5μg/mL阿奇霉素共同作用于PAO1时,能够增强阿奇霉素的抑菌效果。白藜芦醇以群体感应抑制机制对铜绿假单胞菌PAO1中生物膜、胞外多糖、绿脓素产生发挥抑制作用。

Pseudomonas aeruginosa regulates the expression of toxic factors such as biofilm,extracellular polysaccharide and pyocyanin by quorum sensing mechanism,so the expression of these toxic factors can be reduced or inhibited by quorum sensing inhibition principle.Study the inhibitory effect of resveratrol as quorum sensing inhibitor on the production of bioflm,extracellular polysaccharide and pyocyanin in Pseudomonas aeruginosa PAO1.It was investigated that the inhibitory effect of resveratrol on the production of purple pigment by Chromobacterium violaceum CVO26,then production of the bioflm,extracellular polysaccharide,pyocyanin of Pseudomonas aeruginosa PAO1 by quorum sensing regulation traits,and the synergistic effect of resveratrol on Pseudomonas aeruginosa PAO1 with azithromycin were determinated.The results showed that there was no growth inhibition to CVO26,but the production of purple pigment of CVO26 was inhibited obviously at resveratrol concentrations of 40 and 60μg/mL.The productions of biofilm,extracellular polysaccharide,and pyocyanin were reduced at the resveratrol concentration of 20-80μg/mL ineffective on Pseudomonas aeruginosa PAO1 growth,and 40μg/mL resveratrol had synergistic inhibitory effect with 5μg/mL azithromycin on PAO1.Resveratrol as a quorum sensing inhibitor can inhibit the expression of biofilm,extracellular polysaccharide and pyocyanin in Pseudomonas aeruginosa PAO1.