RNAscope技术检测hTERC RNA组分在宫颈鳞状上皮内瘤变中的表达及其应用价值

Expression of hTERC RNA Fraction in Cervical Squamous Intraepithelial Neoplasia by RNAscope Technique and Its Application Value

目的:探讨RNAscope技术检测hTERC RNA组分表达对宫颈鳞状上皮内瘤变分级诊断价值。方法:筛选2021年9月至2022年2月华北理工大学附属医院病理科已确诊为HR-HPV感染的宫颈活检标本80例,分3组:炎症组14例、低级别组34例、高级别组32例。分别采用荧光原位杂交技术及RNAscope技术检测石蜡包埋组织中hTERC基因的扩增状况及其RNA组分的表达。结果:FISH检测hTERC基因扩增三组间具有差异性(P<0.05)。RNAscope检测三组间检出率具有统计学差异(P<0.05)。组织学诊断分组中,炎症组14例中hTERC RNA组分阳性表达程度均为(-/++),低级别组中以hTERC RNA组分(++)为主(19/34),高级别组中以hTERC RNA组分(+++/++++)为主(27/32),上述染色模式表达情况在组织学分级诊断中具有统计学差异(P<0.05)。结论:RNAscope技术检测宫颈上皮内瘤变组织中hTERC RNA组分的表达有助于宫颈病变组织学诊断及进展预测。

Objective:To investigate the diagnostic significance of hTERC RNA fractions expression detected by RNA scope technique in cervical squamous intraepithelial neoplasia.Methods:A total of 80 cervical biopsy specimens diagnosed with HR-HPV infection in the Department of Pathology,Affiliated Hospital of North China University of Science and Technology from September 2021 to February 2022 were screened in 3 groups:inflammation group(14 cases),low-grade group(34 cases),and high-grade group(32 cases).The amplification status of hTERC gene and the expression of its RNA components in paraffin-embedded tissues were detected by fluorescence in situ hybridization and RNAscope techniques,respectively.Results:FISH detection of hTERC gene amplification was different among the three groups(P<0.05).Detection rate by RNAscope was statistically different among the three groups(P<0.05).In the histological diagnostic subgroups,the degree of positive expression of hTERC RNA fraction was(-/++)in all 14 cases in the inflammation group,the hTERC RNA fraction(++)was predominant in the low-grade group(19/34),and the hTERC RNA fraction(++++/++++)was predominant in the high-grade group(27/32),and the expression of the above staining patterns in the histological graded diagnosis had statistical differences(P<0.05).Conclusion:The RNAscope technique to detect the expression of hTERC RNA fraction in cervical intraepithelial neoplasia tissue helps in the histological diagnosis and progression prediction of cervical lesions.