摘要
目的:探讨RNAscope技术检测hTERC RNA组分表达对宫颈鳞状上皮内瘤变分级诊断价值。方法:筛选2021年9月至2022年2月华北理工大学附属医院病理科已确诊为HR-HPV感染的宫颈活检标本80例,分3组:炎症组14例、低级别组34例、高级别组32例。分别采用荧光原位杂交技术及RNAscope技术检测石蜡包埋组织中hTERC基因的扩增状况及其RNA组分的表达。结果:FISH检测hTERC基因扩增三组间具有差异性(P<0.05)。RNAscope检测三组间检出率具有统计学差异(P<0.05)。组织学诊断分组中,炎症组14例中hTERC RNA组分阳性表达程度均为(-/++),低级别组中以hTERC RNA组分(++)为主(19/34),高级别组中以hTERC RNA组分(+++/++++)为主(27/32),上述染色模式表达情况在组织学分级诊断中具有统计学差异(P<0.05)。结论:RNAscope技术检测宫颈上皮内瘤变组织中hTERC RNA组分的表达有助于宫颈病变组织学诊断及进展预测。
Objective:To investigate the diagnostic significance of hTERC RNA fractions expression detected by RNA scope technique in cervical squamous intraepithelial neoplasia.Methods:A total of 80 cervical biopsy specimens diagnosed with HR-HPV infection in the Department of Pathology,Affiliated Hospital of North China University of Science and Technology from September 2021 to February 2022 were screened in 3 groups:inflammation group(14 cases),low-grade group(34 cases),and high-grade group(32 cases).The amplification status of hTERC gene and the expression of its RNA components in paraffin-embedded tissues were detected by fluorescence in situ hybridization and RNAscope techniques,respectively.Results:FISH detection of hTERC gene amplification was different among the three groups(P<0.05).Detection rate by RNAscope was statistically different among the three groups(P<0.05).In the histological diagnostic subgroups,the degree of positive expression of hTERC RNA fraction was(-/++)in all 14 cases in the inflammation group,the hTERC RNA fraction(++)was predominant in the low-grade group(19/34),and the hTERC RNA fraction(++++/++++)was predominant in the high-grade group(27/32),and the expression of the above staining patterns in the histological graded diagnosis had statistical differences(P<0.05).Conclusion:The RNAscope technique to detect the expression of hTERC RNA fraction in cervical intraepithelial neoplasia tissue helps in the histological diagnosis and progression prediction of cervical lesions.
作者
胡倩岚
张爽
李红娜
毛昕
刘芳
吴晨阳
宋旭东
HU Qianlan;ZHANG Shuang;LI Hongna(The Affiliated Hospital of North China University of Technology,Hebei Tangshan 063000,China)
出处
《河北医学》
CAS
2023年第6期928-932,共5页
Hebei Medicine
基金
2018年度河北省医学适用技术跟踪项目,(编号:G2018067)。
关键词
RNAscope技术
荧光原位杂交
宫颈上皮内瘤变
人端粒酶RNA
RNAscope technique
Fluorescence in situ hybridization
Cervical squamous intraepithelial neoplasia
Human telomerase RNA