帕金森病患者中miR-146a表达意义及与炎症因子的相关性分析

Significance of miR-146a expression in Parkinson's patients and analysis of its correlation with inflammatory factors

目的 探讨帕金森病(PD)患者血清miR-146a表达水平的变化,分析其与炎症因子的相关性。方法 选取我院2017年8月~2021年3月收治的PD患者108例作为PD组,同时纳入同期体检的健康志愿者87例作为对照组。比较两组血清miR-146a表达量及白介素-1β(IL-1β)、IL-2、IL-6、超敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)水平。根据患者miR-146a表达量分成高表达组、低表达组,分析其表达与PD临床特征的关系。绘制受试者工作特征曲线(ROC)分析血清miR-146a评估PD的曲线下面积(AUC)。经Pearson线性相关分析血清miR-146a表达水平与炎症因子的相关性。结果 PD组血清miR-146a表达低于对照组,血清IL-1β、IL-2、IL-6、TNF-α、hs-CRP水平高于对照组(P<0.05)。miR-146a低表达组UPDRS评分≥50分、H-Y 3~5级、认知障碍占比高于高表达组(P<0.05)。血清miR-146a表达评估PD的AUC为0.795。Pearson线性相关分析提示血清miR-146a表达与IL-1β、IL-2、IL-6、TNF-α、hs-CRP水平呈负相关(P<0.05)。结论 PD患者血清miR-146a表达水平下调,且与炎症因子密切相关,其对PD诊断有一定价值。

Objective To explore the changes of serum miR-146a expression level in patients with Parkinson's disease(PD),and analyze its correlation with inflammatory factors.Methods A total of 108 PD patients admitted to our hospital from August 2017 to March 2021 were selected as the PD group,and 87 healthy volunteers were included as the control group.The serum miR-146a expression and the levels of interleukin-1β(IL-1β),IL-2,IL-6,high-sensitivity C-reactive protein(hs-CRP) and tumor necrosis factor-α(TNF-α) were compared between the two groups.According to the expression of miR-146a,the patients were divided into high expression group and low expression group,and the relationship between its expression and the clinical characteristics of PD was analyzed.ROC was drawn to analyze the AUC of serum miR-146a to assess PD.The correlation between serum miR-146a expression level and inflammatory factors was analyzed by Pearson linear correlation.Results The expression of serum miR-146a in the PD group was lower than control group,and the levels of serum IL-1β,IL-2,IL-6,TNF-α,and hs-CRP were higher than those in the control group(P<0.05).The UPDRS score of the miR-146a low expression group was ≥50 points,H-Y grade 3 to 5,and the proportion of cognitive impairment was higher than the high expression group(P<0.05).Serum miR-146a expression assessed PD AUC of 0.795.Pearson linear correlation analysis showed that serum miR-146a expression was negatively correlated with IL-1β,IL-2,IL-6,TNF-α,and hs-CRP levels(P<0.05).Conclusion The expression level of serum miR-146a in PD patients is down-regulated and is closely related to inflammatory factors,which has a certain value in the diagnosis of PD.