骆驼乳清蛋白对对乙酰氨基酚致小鼠肝损伤的保护作用

Protective Effect of Camel Whey Protein on Liver Injury Induced by Acetaminophen in Mice

本试验旨在研究骆驼乳清蛋白(CWP)对对乙酰氨基酚(APAP)所致小鼠肝损伤的保护作用。选取6周龄KM小鼠,适应性饲养1周后,随机分成6组,每组8只。正常对照组(NC)和APAP致肝损伤模型组(LD)的小鼠饲喂基础日粮并每天灌胃生理盐水,连续处理8 d后,LD组小鼠经腹腔注射APAP(325 mg/kg);N-乙酰半胱氨酸阳性药物对照组(NAC)的小鼠,每天腹腔注射N-乙酰半胱氨酸(150 mg/kg),连续处理8 d后,经腹腔注射APAP(325 mg/kg);CWP低、中、高剂量组(LCWP、MCWP和HCWP)的小鼠,每天分别灌胃100 mg/kg、200 mg/kg和400 mg/kg CWP,连续处理8 d后经腹腔注射APAP(325 mg/kg),造成小鼠急性肝损伤。注射APAP后12 h,各组小鼠经眼球采血并分离血清后处死。采集肝组织经H&E染色后观察组织学变化;检测小鼠血清中肝功能生物标志物水平、氧化应激标志物水平及肝脏中的抗氧化酶活性。结果表明:与LD组相比,低、中、高剂量CWP干预均能显著降低小鼠血清丙氨酸氨基转移酶活力、天冬氨酸氨基转移酶活力和肿瘤坏死因子-α含量(P<0.05),显著增强超氧化物歧化酶活性(P<0.05);中、高剂量CWP可显著降低小鼠血清丙二醛和白介素-6含量(P<0.05);高剂量CWP可有效缓解APAP所致的小鼠肝组织病理学改变,显著提高血清谷胱甘肽水平(P<0.05)。由此可见,CWP可增强小鼠肝脏的抗氧化能力,并抑制炎症反应,最终缓解了APAP诱导的肝损伤。

This paper aimed to investigate the protective effect of camel whey protein on acetaminophen-induced liver injury in mice.Six-week-old KM mice were selected and randomly divided into 6 groups after adaptive feeding for one week,with eight mice in each group.Mice in the normal control group(NC)and APAP-induced liver injury model group(LD)were fed the basal diet and given normal saline daily by the gavage method.After continuous treatment for eight days,mice in the LD group were treated with APAP(325 mg/kg)intraperitoneally.Mice in the N-acetylcysteine-positive drug control group(NAC)were treated daily with N-acetylcyste-ine(150 mg/kg)intraperitoneal injection for eight days,followed by the APAP administration.Mice in the CWP low-,medium-,and high-dose groups(LCWP,MCWP and HCWP)were treated daily with 100 mg/kg,200 mg/kg,and 400 mg/kg CWP by intraperitoneal injections of APAP after continuous treatment for eight days.At twelves hours after APAP administration,the serum was separated from eyeball blood of mice in each group and sacrificed.The liver tissue was quickly collected and fixed,and the histological changes were observed after H&E staining.The contents of liver function biomarkers and oxidative stress markers in mice serum,including the activities of antioxidant enzymes in the liver,were detected.The results showed that three doses of CWP could significantly re-duce the activity of alanine aminotransferase,aspartate aminotransferase and the content of tumor necrosis factor-αin the serum of mice with liver injury(P<0.05),and significantly enhance the activity of superoxide dismutase(P<0.05).Medium and high doses of CWP significantly reduced serum malondialdehyde and interleukin-6 levels in mice(P<0.05).High-dose CWP could effectively alleviate APAP-induced liver histopathological changes in mice and significantly increase serum glutathione levels(P<0.05).It was observed that CwP intervention can alleviate the pathological changes of liver tissue in mice in a dose-dependent manner,enhance the antioxidant defense ability of mice and inhibit the production of major inflammatory factors,thereby alleviating APAP-induced liv-er injury in mice.Thus,CWP may enhance the antioxidant capacity and inhibit the inflammatory response in mouse liver,ultimately alleviating APAP-induced liver injury.