红色红曲菌M7的丝衣霉酸基因簇及其功能研究

Study on the byssochlamic acid gene cluster of Monascus ruber M7 and its function

【目的】明确红色红曲菌(Monascus ruber) M7的基因组中是否存在丝衣霉酸(byssochlamic acid, BA)基因簇,并探讨BA基因簇中聚酮合酶基因mr-Bys及3-羟酰基辅酶A脱氢酶基因mr-hdh对BA产生的影响。【方法】采用生物信息学方法对M7基因组进行分析,以确定BA候选基因簇;以基因敲除及过表达方法研究mr-Bys和mr-hdh对BA产生的影响;以超高效液相色谱(ultra-performance liquid chromatography, UPLC)和质谱(mass spectrometry, MS)方法分析BA。【结果】在红色红曲菌M7基因组中发现了BA基因簇,敲除mr-Bys后,BA消失,而敲除和过表达mr-hdh均可影响BA的产生。【结论】红色红曲菌M7基因组中存在BA基因簇,可产生BA。研究结果不仅丰富了红曲菌次生代谢产物及其合成途径的相关研究,也为以红曲菌为菌种开发新产品奠定了基础。

[Objective]To determine whether the byssochlamic acid(BA)gene cluster exists in the genome of Monascus ruber M7,and to explore the effect of polyketide synthase gene mr-Bys and 3-hydroxyacyl-CoA dehydrogenase gene mr-hdh in BA gene cluster on BA production.[Methods]The M7 genome was analyzed by bioinformatics methods to identify the BA candidate gene clusters.The effects of mr-Bys and mr-hdh on BA production were studied by the gene knockout and over-expression methods.BA was analyzed by ultra-performance liquid chromatography(UPLC)and mass spectrometry(MS).[Results]A BA gene cluster was found in the genome of M.ruber M7.After knocking out mr-Bys,BA disappeared,while both knockout and over-expression of mr-hdh affected BA production by M.ruber M7.[Conclusion]There is a BA gene cluster in the genome of M.ruber M7,which can produce BA.The research results enrich the research on the secondary metabolites of Monascus spp.and their synthetic pathways and lay a foundation for the development of new products using Monascus spp.