UHPLC-MS/MS法检测化妆品中禁用原料非那西丁

Detection of Prohibited Ingredient Phenacetin in Cosmetics with UHPLC-MS/MS

建立了灵敏检测化妆品中禁用原料非那西丁的超高效液相色谱-三重四极杆串联质谱(UHPLC-MS/MS)法。针对染发剂、烫发剂、膏霜、乳液和液态水基样品,用饱和氯化钠分散,乙腈作为提取溶剂进行超声辅助液液萃取。采用Poroshell 120 EC-C_(18)(100 mm×2.1 mm×2.7μm)色谱柱,水和乙腈分别作为流动相。串联质谱检测器采用ESI源,在多反应监测(MRM)模式下进行检测,并进行了方法评价。该方法线性关系良好,在5种基质中的检出限为0.002~0.006 mg/kg,定量限为0.006~0.02 mg/kg,加标回收率为93.7%~113.7%,相对标准偏差(RSD)为1.0%~5.6%。对60批化妆品进行检测,其中3批染发剂样品和1批烫发剂样品中检出非那西丁。该方法操作简便、灵敏度高,适用于化妆品中禁用原料非那西丁的准确检测。

An ultra performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)method was developed for the sensitive detection of phenacetin,a prohibited ingredient in cosmetics.The hair dye,perming formula,cream,lotion and toner samples were firstly dispersed with saturated NaCl solution,and then acetonitrile was added for ultrasound-assisted liquid-liquid extraction.Separation was carried out on a Poroshell 120 EC-C_(18) column(100 mm×2.1 mm×2.7μm).Water and acetonitrile were used as liquid phases,respectively.A tandem mass spectrometry detector with ESI source in multiple reaction monitoring(MRM)mode was employed for detection.This analysis method was finally performed and evaluated in detail.The method showed good linearity with the limits of detection(LOD)of 0.002-0.006 mg/kg and the limits of quantification(LOQ)of 0.006-0.02 mg/kg in five kinds of sample matrix.The spiked recoveries ranged from 93.7%to 113.7%,and the relative standard deviation(RSD)was 1.0%-5.6%.This method was applied to 60 batches of cosmetics samples,among which 3 batches of hair dye samples and one batch of perming formula product were detected with phenacetin.This method is simple and sensitive.It is suitable for the accurate detection of phenacetin,a prohibited ingredient in cosmetics.