外植体取样时间和消毒方式对沙子空心李诱导培养的影响

Effects of Explant Sampling Time and Disinfection Methods on Induction Culture of Shazi Kongxin Plum

为研究外植体取样时间和消毒方式对沙子空心李诱导培养的影响,本试验以不同时间段取样的沙子空心李半木质化茎段为外植体,分别采用2种消毒方式处理后进行诱导培养。结果表明:与2%次氯酸钠消毒相比,使用0.1%氯化汞进行消毒时,外植体污染率较低、成活率较高;随着外植体取样时间的推移,诱导死亡率总体上逐渐降低,4月25日取样的外植体死亡率最低、成活率最高。综合分析可知,沙子空心李外植体适宜的消毒方式是75%乙醇消毒10 s+0.1%氯化汞消毒5 min,适宜的取样时间是4月下旬。该条件下,外植体诱导污染率达到最低(4%),成活率达到最高(86%)。

To study the effects of explant sampling time and disinfection methods on induction culture of Shazi Kongxin plum,this experiment used semi-lignified stem segments of Shazi Kongxin plum sampled at different time periods as explants,and treated them with two disinfection methods before induction culture.The results showed that compared with 2%NaClO disinfection,using 0.1%HgCl2 for disinfection resulted in a lower pollution rate and a higher survival rate of the explants.As the sampling time of the explants progressed,the induced mortality rate gradually decreased overall.The explants sampled on April 25th had the lowest mortality rate and the highest survival rate.Comprehensive analysis showed that the appropriate disinfection method for Shazi Kongxin plum was 75%ethanol disinfection for 10 seconds and 0.1%HgCl2 disinfection for 5 minutes,the appropriate sampling time was late April.Under this condition,the induced pollution rate of explants was the lowest(4%),the survival rate reached the highest(86%).