金银花WRKY基因家族的鉴定与功能分析

Identification and Functional Analysis of WRKY Gene Family of Lonicera japonica Thunb

WRKY转录因子参与调控植物的生长发育、激素应答及逆境应答等过程.基于金银花的基因组数据信息鉴定了WRKY基因家族成员,并分析了WRKY转录因子序列的保守结构域、启动子特点、进化特征、基因重复情况以及在两组不同转录组中的表达情况.结果表明:金银花共有46个WRKY基因,从进化特点上可分为3个亚家族,基因数量分别为16,13,17个.WRKY家族成员的蛋白分子量在15 963.55~80 967.23 Da之间,氨基酸数量在149~748之间,等电点在4.75~9.71之间,不稳定系数在40.97~67.76之间.参与生长发育、激素应答的元件出现在金银花WRKY基因的启动子区域.通过分析两组不同的表达谱数据发现,WRKY转录因子可能参与了调控金银花的开花过程.研究为进一步开展金银花WRKY家族基因的功能研究奠定了基础.

WRKY transcription factors play an important role in plants,and they participate in the regulation of plant growth and development,hormone and stress response.In this study,the WRKY gene family was identified based on the genomic information of Lonicera japonica,and the conserved domain,promoter characteristics,evolutionary characteristics,gene duplication and expression of WRKY transcription factors in two different transcriptomes were analyzed.Finally,46 WRKY genes were identified from Lonicera japonica,which could be divided into three subfamilies:Ⅰ(16),Ⅱ(13),andⅢ(17)by evolutionary analysis.The molecular weight of WRKY proteins ranged from 15963.55 to 80967.23 Da,the number of amino acids ranged from 149 to 748,the isoelectric point ranged from 4.75 to 9.71,and the instability coefficient ranged from 40.97 to 67.76.Many elements involved in growth and development,and hormone response present in the promoter region of WRKY in Lonicera japonica.By analyzing two different expression profiles,we found that WRKY transcription factors might regulate the flowering process.Our research will lay a foundation for further functional studies of WRKY genes in Lonicera japonica.