黑色素瘤相关抗原A3 E-钙黏蛋白及P53在宫颈癌组织中的表达与意义

Expressions and significance of melanoma-associated antigen-A3,E-cadherin,and P53 in cervical cancer tissue

目的研究黑色素瘤相关抗原A3(MAGE-A3)、E-钙黏蛋白(E-cad)及P53在宫颈癌组织中的表达与意义,为宫颈癌基因方面的病情检测提供新的思路。方法选取2019年3月—2021年3月义乌市中心医院收治的86例宫颈癌患者为研究对象,应用免疫组织化学法检测患者癌组织及相应的癌旁组织中MAGE-A3、E-cad、P53的表达水平,采用Pearson相关性分析法分析MAGE-A3、E-cad、P53之间的相关性。结果宫颈癌组织中MAGE-A3、E-cad、P53阳性表达率分别为45.35%(39例)、40.70%(35例)、26.74%(23例);癌旁组织中MAGE-A3、E-cad、P53阳性表达率分别为8.14%(7例)、88.37%(76例)、2.33%(2例)。与癌旁组织比较,癌组织中MAGE-A3、P53阳性表达率均升高,E-cad阳性表达率降低,两组比较差异均具有统计学意义(MAGE-A3:χ^(2)=30.388,P<0.05;E-cad:χ^(2)=42.702,P<0.05;P53:χ^(2)=18.721,P<0.05)。不同TNM分期、分化程度、淋巴结转移情况的患者宫颈癌组织中MAGE-A3、E-cad、P53阳性表达率比较,差异均具有统计学意义(均P<0.05)。宫颈癌组织中,MAGE-A3与E-cad表达呈负相关(r=-0.261,P<0.05),MAGE-A3与P53表达呈正相关(r=0.871,P<0.05),E-cad与P53表达呈负相关(r=-0.486,P<0.05)。结论宫颈癌组织中MAGE-A3、P53阳性表达呈上调状态,E-cad阳性表达呈下调状态,三者之间紧密相关,并且其表达水平的高低变化与TNM分期、分化程度、淋巴结转移等临床病理特征有关,可能共同参与了宫颈癌的发生、发展。

Objective To study the expressions and significance of melanoma-associated antigen-A3(MAGE-A3),E-cadherin(E-cad),and P53 in cervical cancer,provide a new idea for gene detection of cervical cancer.Methods A total of 86 patients with cervical cancer were selected from Yiwu Central Hospital from March 2019 to March 2021 as study object,the expression levels of MAGE-A3,E-cad,and P53 in cancer tissues and corresponding paracancerous tissues of these patients were analyzed by immunohistochemistry.The corre-lations of the three indexes were analyzed by Pearson correlation analysis method.Results The positive expression rates of MAGE-A3,E-cad,and P53 in cervical cancer tissue were 45.35%(39 cases),40.70%(35 cases),and 26.74%(23 cases),respectively;the posi-tive expression rates of MAGE-A3,E-cad,and P53 in adjacent tissue of cervical cancer were 8.14%(7 cases),88.37%(76 cases),and 2.33%(2 cases),respectively.Compared with adjacent tissue of cervical cancer,the positive expression rates of MAGE-A3 and P53 in cervical cancer tissue increased,the positive expression rate of E-cad in cervical cancer tissue decreased,there were statistically significant differences(P<0.05).There were statistically significant differences in the positive expression rates of MACE-A3,E-cad,and P53 in cervical cancer tissue of the patients with different TNM stages,differentiation degrees,and lymph node metastasis(MAGE-A3:X?30.388,P<0.05;E-cad:X^(2)=42.702,P<0.05;P53:X^(2)=18.721,P<0.05).In cervical cancer tissue,MAGE-A3 expression was neg-atively correlated with E-cad expression(r=-0.261,P<0.05),MAGE-A3 expression was positively correlated with P53 expression(r=0.871,P<0.05),E-cad expression was negatively correlated with P53 expression(r=-0.486,P<0.05).Conclusion The positive ex-pressions of MAGE-A3 and P53 in cervical cancer tissue are up-regulated,and the positive expression of E-cad in cervical cancer tissue is down-regulated.There are close correlations among the three indexes,and the expression levels are correlated with TNM stage,degree of differentiation,and lymph node metastasis,which may be involved in the occurrence and development of cervical cancer.