罗伊氏乳杆菌FN041对高脂高果糖喂养小鼠糖脂代谢的影响

EFFECT OF LACTOBACILLUS REUTERI FN041 ON GLYCOLIPID METABOLISM IN MICE FED A HIGH-FAT AND HIGH-FRUCTOSE DIET

目的探讨罗伊氏乳杆菌FN041对高脂高果糖喂养小鼠糖脂代谢紊乱的改善作用。方法SPF级5w龄雄性C57小鼠32只,随机分为4组,每组8只:正常对照组(controldiet,CD)喂正常饲料+饮纯净水+纯净水灌胃;高脂高果糖模型组(highfathighfructosediet,HFFD)喂高脂饲料+饮10%果糖水+纯净水灌胃;鼠李糖乳杆菌组(Lactobacillus rhamnosus GG,LGG)喂高脂饲料+饮10%果糖水+LGG(1×10^(9)CFU/d)灌胃;罗伊氏乳杆菌FN041组(Lactobacillus reuteri FN041)喂高脂饲料+10%果糖水+FN041(1×10^(9)CFU/d)灌胃。喂养8 w后处死小鼠,称量小鼠体重、肝重、附睾脂肪及肾周脂肪总重、计算肝脏指数;用全自动生化分析仪检测血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、空腹血糖(fasting blood glucose,FBG)水平;采用放免法测定血清胰岛素水平(insulin level,INS),计算胰岛素抵抗指数(insulinresistanceindex,HOMA-IR)。结果相比CD组,HFFD组小鼠体重、附睾脂肪及肾周脂肪总重、肝重、血清TG、TC、HDL-C、FBG、INS水平、HOMA-IR均显著升高,肝脏指数明显降低(P<0.05)。与HFFD组相比,LGG组小鼠体重、肝重、血清TG、FBG水平均显著降低(P<0.05);FN041组小鼠体重、附睾脂肪及肾周脂肪总重、肝重、血清ALT、FBG、INS、HOMA-IR均显著降低(P<0.05)。此外,与LGG组相比,FN041组小鼠血清FBG水平降低了23%,且差异有统计学意义(P<0.05)。结论罗伊氏乳杆菌FN041可改善高脂高果糖喂养小鼠的糖代谢紊乱,减轻胰岛素抵抗。[营养学报,2023,45(2):188-192]

Objective To investigate the effects of Lactobacillus reuteri FN041 on glucolipid metabolism disorder induced by a high fat and high fructose diet in mice.Methods Thirty-two 5-week-old male SPF C57 mice were randomly divided into four groups(n=8/group).The control group(CD)was fed the control diet plus purified drinking water and gavaged with purified water.The high fat high fructose model group(HFFD)was fed the high-fat diet plus 10%fructose drinking water and gavaged with purified water.The Lactobacillus rhamnosus GG group(LGG)was fed the high-fat diet plus 10%fructose drinking water and gavaged with LGG bacteria(1×10^(9)CFU/day).The Lactobacillus reuteri FN041 group(FN041)was fed the high-fat diet plus 10%fructose drinking water and gavaged with FN041 bacteria(1×10^(9) CFU/day).After 8 weeks of feeding,the mice were sacrificed.The body weight,fat weight and liver weight were weighed,and the liver index was calculated.Triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and fasting blood glucose(FBG)in the serum were detected by an automatic biochemical analyzer.Serum insulin(INS)was detected by radioimmunoassay,and the homeostasis model insulin resistance index(HOMA-IR)was calculated.Results Compared with the CD group,the body weight,total weight of epididymal fat and perirenal fat,liver weight,serum TG,TC,HDL-C,FBG,INS and HOMA-IR of the HFFD group were significantly increased,and liver index was markedly decreased(P<0.05).In comparison with the HFFD group,the body weight,liver weight,the levels of serum TG and FBG were significantly decreased in the LGG group(P<0.05).The body weight,total weight of epididymal and perirenal fats,liver weight,serum levels of ALT,FBG,INS and HOMA-IR in the FN041 group were significantly decreased(P<0.05).In addition,compared with the LGG group,serum FBG level of the FN041 group was decreased by 23%(P<0.05).Conclusion Lactobacillus reuteri FN041 can improve the disorder of glucose metabolism and alleviate insulin resistance in mice fed the high-fat and high-fructose diet.[ACTA NUTRIMENTA SINICA,2023,45(2):188-192]