人参皂苷Rb1对MPTP诱导的帕金森病模型小鼠神经保护作用的初探

Preliminary study on neuroprotective effect of Ginsenoside Rb1 on MPTP-induced Parkinson's disease model mice

目的:探讨人参皂苷Rb1(GsRb1)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)模型小鼠的神经保护作用及量效关系。方法:将50只C57BL/6小鼠随机分为5组,分别为对照组、MPTP组、GsRb1处理组(低剂量组、中剂量组和高剂量组),每组10只。模型组和处理组使用MPTP(30 mg/kg)连续腹膜腔注射5 d制备PD小鼠模型。处理组每天分别以10 mg/kg、20 mg/kg和40 mg/kg的剂量腹膜腔注射GsRb1;对照组给予等容量生理盐水。给药结束后第2 d,采用转棒实验、爬杆实验、悬挂实验等行为学方法检测小鼠的行为学变化。结果:与对照组相比,MPTP组小鼠掉落潜伏期显著降低(P<0.01),爬杆时间显著升高(P<0.01),悬挂实验评分显著降低(P<0.01)。与MPTP组比较,中、高剂量组掉落潜伏期明显升高(P<0.05);低、中剂量组爬杆时间明显降低(P<0.05);中、高剂量组悬挂实验评分明显升高(P<0.05)。结论:GsRb1对MPTP诱导的PD模型小鼠具有神经保护作用,每天给予20 mg/kg效果较好。

Objective:To investigate the neuroprotective effect of ginsenoside Rb1(GsRb1)on Parkinson's disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)and its dose-effect relationship.Methods:Fifty C57BL/6 mice were randomly divided into five groups:control group,MPTP group,GsRb1 treatment group(low-dose group,middle dose group and high-dose group),with 10 mice in each group.The model group and the treatment group were injected MPTP(30 mg/kg)into the peritoneal cavity continuously for 5 days to prepare the PD mouse model.The treatment group received intraperitoneal injection of GsRb1 at doses of 10 mg/kg,20 mg/kg and 40mg/kg respectively every day.The control group was given the same amount of normal saline.On the second day after administration,the behavioral changes of the mice were detected by using Rotarod test,Pole-climbing test and Grasping test.Results:Compared with the control group,the latent period of MPTP group mice was significantly reduced(P<0.01),the climbing time was significantly increased(P<0.01),and the Graspiing test score was significantly reduced(P<0.01).Compared with the MPTP group,the latent period in the middle and high dose groups increased significantly(P<0.05),the climbing time in low and middle dose groups decreased significantly(P<0.05)and the scores of Graspiing test in middle and high dose groups were significantly higher(P<0.05).Conclusion:GsRb1 has neuroprotective effect on MPT-induced PD model mice,and 20 mg/kg/d has a better effect.